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Abstract

This study aimed to evaluate the cytotoxic effects of Acrylamide (ACR) on the pituitary gland and bone marrow of male rats. A total of 40 male rats were used and randomly divided into four groups, each including 12 rats. The first and second groups were considered as control and were kept for 6 and 8 weeks. The third group was administrated orally with Acrylamide at two doses/week as 4mg/kg.b.w for 6 weeks. The fourth group was administrated orally with Acrylamide at two doses/week of 4mg/kg.b.w for 8 weeks. After the treatment period, the rats were sacrificed, then their pituitary glands were removed and processed for histological examinations. Histological examination showed degeneration, congestion of the blood vessels, deposition of collagen fibers, heavy infiltration of inflammatory cells, and having pitucyte embedded with collagen. After 8 weeks post-treatment, the results showed pitucytes with vaculated cytoplasm, necrosis with degenerated pitucytes, irregular distribution of pitucytes nuclei and hemorrhage.  Pituitary sections stained with PAS stain in control rats showed the homogenous distribution of the mucopoly saccharides and all cells within anterior and posterior regions. While sections of rats after 8 weeks post-treatment showed the homogenous distribution of the mucopoly saccharides in the posterior and moderate in the anterior regions. The results of chromosomal aberration in male rats’ bone marrow cells treated with 4 mg/kg.bw after 8 weeks showed dicentric chromosomes, fragment centromeric separation, and ring chromosomes. The results showed a significant increase in serum T4 and a decrease in serum TSH level of groups exposed to acrylamide in both 6 and 8 weeks periods compared with control groups.

Keywords

Acrylamide Pituitary gland TSH T4 Chromosomes aberration

Article Details

How to Cite
NADHIM, A. A. ., & AL-DERAWI, K. H. (2022). Histological, biochemical and chromosomal aberrations of pituitary gland induced by acrylamide in male rats. Iranian Journal of Ichthyology, 9, 412–423. Retrieved from http://www.ijichthyol.org/index.php/iji/article/view/840 (Original work published July 1, 2022)

References

  1. Agarwal, A.; Virk, G.; Ong, C. & Du Plessis, S.S. 2014. Effect of oxidative stress on male reproduction. The World Journal of Men's Health 32(1): 1-17.
  2. Adler, I.D.; Baumgartner, A.; Gonda, H.; Friedman, M.A. & Skerhut, M. 2000. Aminobenzotriazole inhibits acrylamide-induced dominant lethal effects in spermatids of male mice. Mutagenesis 15(2): 133-136.
  3. ‏‏‏‏‏‏‏Al-Okaily, B.N. 2017. Histological changes in pituitary-testes axis in rats exposed to cadmium chloride: Protective role of Eruca sativa seeds. The Iraqi Journal of Veterinary Medicine 41(1): 76-85.
  4. Bajilan, S.I. & Al-naqeeb, A.A. 2011. Effect of the aqueous extract of rocket (Eruca sativa L.) leaves on the histological structure of some organs in male mice. Journal of the College of Basic Education 17(70): 13-21.‏
  5. Baum, M.; Loeppky, R.N.; Thielen, S. & Eisenbrand, G. 2008. Genotoxicity of glycidamide in comparison to 3-N-nitroso-oxazolidin-2-one. Journal of Agricultural and Food Chemistry 56(15): 5989-5993.
  6. Borgeest, C.; Greenfeld, C.; Tomic, D. & Flaws, J.A. 2002. The effects of endocrine disrupting chemicals on the ovary. Frontiers in Bioscience 7(1-3): d1941-d1948.
  7. Brisson, B.; Ayotte, P.; Normandin, L.; Gaudreau, É.; Bienvenu, J.F.; Fennell, T.R. & Bouchard, M. 2014. Relation between dietary acrylamide exposure and biomarkers of internal dose in Canadian teenagers. Journal of Exposure Science & Environmental Epidemiology 24(2): 215-221. ‏‏Cano, P.; Poliandri, A. H.; Jiménez, V.; Cardinali, D.P. & Esquifino, A.I. 2007. Cadmium-induced changes in Per 1 and Per 2 gene expression in rat hypothalamus and anterior pituitary: effect of melatonin. Toxicology Letters 172(3): 131-136.
  8. Capen, C.C. 1983. Functional and pathologic interrelationships of the pituitary gland and the hypothalamus. In Endocrine System (pp. 101-120). Springer, Berlin, Heidelberg.
  9. ‏Dearfield, K.L.; Douglas, G.R.; Ehling, U.H.; Moore, M.M.; Sega, G.A. & Brusick, D.J. 1995. Acrylamide: a review of its genotoxicity and an assessment of heritable genetic risk. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 330(1-2): 71-99.
  10. Yeşim, Y.; Fatma Hümeyra, Y. & Aysun, T. 2016. Evaluation of Some Renal Function Parameters in Rats Treated with Acrylamide. ARC Journal of Animal and Veterinary Sciences (AJAVS). 2(1): 1-8.
  11. Hammad, A.Y.; Osman, M.E.; & Abdelgadir, W.S. 2013. Effects of acrylamide toxicity on growth performance and serobiochemisty of Wistar rats. British Journal of Pharmacology and Toxicology 4(4): 163-168. ‏
  12. Higashikuni, N.; Hara, M.; Nakagawa, S. & Sutou, S. 1994. 2-(2-Furyl)-3-(5-nitro-2-furyl) acrylamide (AF-2) is a weak in vivo clastogen as revealed by the micronucleus assay. Mutation Research/Genetic Toxicology 320(1-2): 149-156.
  13. Hogervorst, J.G.; Saenen, N.D. & Nawrot, T.S. 2021. Gestational acrylamide exposure and biomarkers of fetal growth: Probing the mechanism underlying the association between acrylamide and reduced fetal growth. Environment International 155: 106668.
  14. Khalil, W.K.B.; Ahmed, H.H.; Hanan, F.; Aly, H. & Eshak, M. 2014. Toxicological effects of acrylamide on testicular function and immune genes expression profile in rats. International Journal of Pharmaceutical Sciences Review and Research 24(1): 143-151.
  15. Khan, M.A.; Davis, C.A.; Foley, G.L.; Friedman, M.A. & Hansen, L.G. 1999. Changes in thyroid gland morphology after acute acrylamide exposure. Toxicological Sciences: An Official Journal of the Society of Toxicology 47(2): 151-157.
  16. Konings, E.J.; Baars, A.J.; van Klaveren, J.D.; Spanjer, M.C.; Rensen, P.M.; Hiemstra, M. & Peters, P. W. J. (2003). Acrylamide exposure from foods of the Dutch population and an assessment of the consequent risks. Food and Chemical Toxicology 41(11): 1569-1579.
  17. Lyon, F. 1994. IARC monographs on the evaluation of carcinogenic risks to humans. Some Industrial Chemicals 60: 389-433.
  18. Mahmood, H. 2014. Anatomical and histological study of pituitary gland of the rats in Iraq. Journal of Kerbala University 10(1): 221-228.
  19. Mannaa, F.; Abdel‐Wahhab, M.A.; Ahmed, H.H. & Park, M.H. 2006. Protective role of Panax ginseng extract standardized with ginsenoside Rg3 against acrylamide‐induced neurotoxicity in rats. Journal of Applied Toxicology: An International Journal 26(3): 198-206.
  20. Matoso, V.; Bargi-Souza, P.; Ivanski, F.; Romano, M.A. & Romano, R.M. 2019. Acrylamide: A review about its toxic effects in the light of Developmental Origin of Health and Disease (DOHaD) concept. Food Chemistry 283: 422-430.
  21. Maan, A.A.; Anjum, M.A.; Khan, M.K.I.; Nazir, A.; Saeed, F.; Afzaal, M. & Aadil, R.M. 2020. Acrylamide formation and different mitigation strategies during food processing–a review. Food Reviews International, 1-18. ‏‏
  22. Nordin-Andersson, M.; Walum, E.; Kjellstrand, P. & Forsby, A. 2003. Acrylamide-induced effects on general and neurospecific cellular functions during exposure and recovery. Cell biology and Toxicology 19(1): 43-51
  23. Pedreschi, F.; Mariotti, M.S. & Granby, K. 2014. Current issues in dietary acrylamide: formation, mitigation and risk assessment. Journal of the Science of Food and Agriculture 94(1): 9-20.
  24. Somasundaram, D.B.; Manokaran, K.; Selvanesan, B.C. & Bhaskaran, R.S. 2017. Impact of di-(2-ethylhexyl) phthalate on the uterus of adult Wistar rats. Human & Experimental Toxicology 36(6): 565-572.‏
  25. Tyl, R.W. & Friedman, M.A. 2003. Effects of cacrylamide on rodent reproductive performance. Reproductive Toxicology 17(1): 1-13.‏
  26. Wang, H.; Huang, P.; Lie, T.; Li, J.; Hutz, R.J.; Li, K. & Shi, F. 2010. Reproductive toxicity of acrylamide-treated male rats. Reproductive Toxicology 29(2): 225-230.‏