Main Article Content
Abstract
The present work was performed to examine the presence of cystic Echinococcus granulosus and Taenia hydatigena in sheep using antigenic based indirect enzyme-linked immunosorbent assay (iELISA) and postmortem analyses. For this purpose, the blood samples of 120 sheep from Afak, Dagharah and Diwaniyah cities were taken. The results showed that, for Afak, 20 and 23 samples were respectively positive for the parasitic antigens and for Dagarah and Diwanyiah, 18 and 22, and 22 and 22 samples were respectively positive for both parasitic antigens. The specificity values recorded were 50 and 63.3%, respectively. In the postmortem examination (PM), the results showed a lower presence of the parasites. Based on the results, ELISA provides a better method for the clinical detection of sheep infected with E. granulosus or C. tenuicollis.
Keywords
Cestode
Echinococcosis
Taenia
ELISA
Article Details
License
The authors transfer the copyrights of their papers to the Iranian Society of Ichthyology. However, the information could be used in accordance with the Creative Commons licence (Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
How to Cite
KARIM, S. M., MAHDEE, N. K., ABBAS, K. H., & MAA, A. (2022). A comparative study of sheep infected with Echinococcus granulosus and Taenia hydatigena using antigenic based ELISA and postmortem analyses. Iranian Journal of Ichthyology, 9, 298–301. Retrieved from http://www.ijichthyol.org/index.php/iji/article/view/821
References
-
Abdulhameed, M.F.; Habib, I.; Al-Azizz, S.A. & Robertson, I. 2018. Cystic echinococcosis in marketed offal of sheep in Basrah, Iraq: Abattoir-based survey and a probabilistic model estimation of the direct economic losses due to hydatid cyst. Parasite Epidemiology and Control 3(1): 43-51.
Agudelo Higuita, N.I.; Brunetti, E. & McCloskey, C. 2016. Cystic echinococcosis. Journal of Clinical Microbiology 54(3): 518-523.
Ali, M.J.; Alfatlawi, M.A.A. & Karawan, A.C. 2018. Molecular identification and phylogenetic-tree analysis of Moniezia species from sheep in Al-Diwaniyah city. Bulletin of the Iraq Natural History Museum 15(2): 131-137.
Al-Mahmoudi, A.H.J.; Al-Nailey, K.G.C.; Ali, M.J.; Alfatlawi, M.A.A. & Al-Saeedi, T.A. 2019. Clinical, Parasitic and Histopathological study of Pigeons infested with Raillietina spp. in Al-Qadisiyah province, Iraq. International Journal of Drug Delivery Technology 9(2): 246-249.
Alvi, M.A.; Ohiolei, J.A.; Li, L.; Saqib, M.; Hussain, M.H. & Tayyab, M.H. 2020. In-house developed ELISA indicates high prevalence of anti-echinococcus granulosus IGG in sheep population-an update from Pakistan. Pathogens 9(11): 1-11.
Brik, K.; Hassouni, T.; Youssir, S.; Baroud, S.; Elkharrim, K. & Belghyti, D. 2018. Epidemiological study of Echinococcus granulosus in sheep in the Gharb plain (North-West of Morocco). Journal of Parasitic Diseases 42(4): 505-510.
Craig, P.; Mastin, A.; van Kesteren, F. & Boufana, B. 2015. Echinococcus granulosus: epidemiology and state-of-the-art of diagnostics in animals. Veterinary Parasitology 213(3-4): 132-148.
Hüttner, M.; Nakao, M.; Wassermann, T.; Siefert, L.; Boomker, J.D.; Dinkel, A. & Ito, A. 2008. Genetic characterization and phylogenetic position of Echinococcus felidis (Cestoda: Taeniidae) from the African lion. International Journal for Parasitology 38(7): 861-868.
Jin, Y.; Anvarov, K., Khajibaev, A.; Hong, S. & Hong, S.T. 2013. Serodiagnosis of echinococcosis by ELISA using cystic fluid from Uzbekistan sheep. The Korean Journal of Parasitology 51(3): 313.
Lymbery, A.J.; Jenkins, E.J.; Schurer, J.M. & Thompson, R.A. 2015. Echinococcus canadensis, E. borealis, and E. intermedius. What's in a name?. Trends in Parasitology 31(1): 23-29.
Nakao, M.; Lavikainen, A.; Yanagida, T. & Ito, A. 2013. Phylogenetic systematics of the genus Echinococcus (Cestoda: Taeniidae). International Journal for Parasitology 43: 1017-1029.
Romig, T.; Ebi, D. & Wassermann, M. 2015. Taxonomy and molecular epidemiology of Echinococcus granulosus sensu lato. Veterinary Parasitology 213(3-4): 76-84.
Scala, A.; Pipia, A.P.; Dore, F.; Sanna, G.; Tamponi, C.; Marrosu, R. & Varcasia, A. 2015. Epidemiological updates and economic losses due to Taenia hydatigena in sheep from Sardinia, Italy. Parasitology Research 114(8): 3137-3143.
Singh, B.B.; Sharma, R.; Gill, J.P.S. & Sharma, J.K. 2015. Prevalence and morphological characterisation of Cysticercus tenuicollis (Taenia hydatigena cysts) in sheep and goat from north India. Journal of Parasitic Diseases 39(1): 80-84.
Tianli, L.; Xifeng, W.; Zhenzhong, T.; Lixia, W.; Xingxing, Z.; Jun, Q. & Xuepeng, C. 2019. Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep. The Korean Journal of Parasitology 57(1): 61.
Valieva, Z.; Sarsembaeva, N.; Valdovska, A. & Ussenbayev, A.E. 2014. Impact of echinococcosis on quality of sheep meat in the South Eastern Kazakhstan. Asian-Australasian Journal of Animal Sciences 27(3): 391.
References
Abdulhameed, M.F.; Habib, I.; Al-Azizz, S.A. & Robertson, I. 2018. Cystic echinococcosis in marketed offal of sheep in Basrah, Iraq: Abattoir-based survey and a probabilistic model estimation of the direct economic losses due to hydatid cyst. Parasite Epidemiology and Control 3(1): 43-51.
Agudelo Higuita, N.I.; Brunetti, E. & McCloskey, C. 2016. Cystic echinococcosis. Journal of Clinical Microbiology 54(3): 518-523.
Ali, M.J.; Alfatlawi, M.A.A. & Karawan, A.C. 2018. Molecular identification and phylogenetic-tree analysis of Moniezia species from sheep in Al-Diwaniyah city. Bulletin of the Iraq Natural History Museum 15(2): 131-137.
Al-Mahmoudi, A.H.J.; Al-Nailey, K.G.C.; Ali, M.J.; Alfatlawi, M.A.A. & Al-Saeedi, T.A. 2019. Clinical, Parasitic and Histopathological study of Pigeons infested with Raillietina spp. in Al-Qadisiyah province, Iraq. International Journal of Drug Delivery Technology 9(2): 246-249.
Alvi, M.A.; Ohiolei, J.A.; Li, L.; Saqib, M.; Hussain, M.H. & Tayyab, M.H. 2020. In-house developed ELISA indicates high prevalence of anti-echinococcus granulosus IGG in sheep population-an update from Pakistan. Pathogens 9(11): 1-11.
Brik, K.; Hassouni, T.; Youssir, S.; Baroud, S.; Elkharrim, K. & Belghyti, D. 2018. Epidemiological study of Echinococcus granulosus in sheep in the Gharb plain (North-West of Morocco). Journal of Parasitic Diseases 42(4): 505-510.
Craig, P.; Mastin, A.; van Kesteren, F. & Boufana, B. 2015. Echinococcus granulosus: epidemiology and state-of-the-art of diagnostics in animals. Veterinary Parasitology 213(3-4): 132-148.
Hüttner, M.; Nakao, M.; Wassermann, T.; Siefert, L.; Boomker, J.D.; Dinkel, A. & Ito, A. 2008. Genetic characterization and phylogenetic position of Echinococcus felidis (Cestoda: Taeniidae) from the African lion. International Journal for Parasitology 38(7): 861-868.
Jin, Y.; Anvarov, K., Khajibaev, A.; Hong, S. & Hong, S.T. 2013. Serodiagnosis of echinococcosis by ELISA using cystic fluid from Uzbekistan sheep. The Korean Journal of Parasitology 51(3): 313.
Lymbery, A.J.; Jenkins, E.J.; Schurer, J.M. & Thompson, R.A. 2015. Echinococcus canadensis, E. borealis, and E. intermedius. What's in a name?. Trends in Parasitology 31(1): 23-29.
Nakao, M.; Lavikainen, A.; Yanagida, T. & Ito, A. 2013. Phylogenetic systematics of the genus Echinococcus (Cestoda: Taeniidae). International Journal for Parasitology 43: 1017-1029.
Romig, T.; Ebi, D. & Wassermann, M. 2015. Taxonomy and molecular epidemiology of Echinococcus granulosus sensu lato. Veterinary Parasitology 213(3-4): 76-84.
Scala, A.; Pipia, A.P.; Dore, F.; Sanna, G.; Tamponi, C.; Marrosu, R. & Varcasia, A. 2015. Epidemiological updates and economic losses due to Taenia hydatigena in sheep from Sardinia, Italy. Parasitology Research 114(8): 3137-3143.
Singh, B.B.; Sharma, R.; Gill, J.P.S. & Sharma, J.K. 2015. Prevalence and morphological characterisation of Cysticercus tenuicollis (Taenia hydatigena cysts) in sheep and goat from north India. Journal of Parasitic Diseases 39(1): 80-84.
Tianli, L.; Xifeng, W.; Zhenzhong, T.; Lixia, W.; Xingxing, Z.; Jun, Q. & Xuepeng, C. 2019. Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep. The Korean Journal of Parasitology 57(1): 61.
Valieva, Z.; Sarsembaeva, N.; Valdovska, A. & Ussenbayev, A.E. 2014. Impact of echinococcosis on quality of sheep meat in the South Eastern Kazakhstan. Asian-Australasian Journal of Animal Sciences 27(3): 391.
Agudelo Higuita, N.I.; Brunetti, E. & McCloskey, C. 2016. Cystic echinococcosis. Journal of Clinical Microbiology 54(3): 518-523.
Ali, M.J.; Alfatlawi, M.A.A. & Karawan, A.C. 2018. Molecular identification and phylogenetic-tree analysis of Moniezia species from sheep in Al-Diwaniyah city. Bulletin of the Iraq Natural History Museum 15(2): 131-137.
Al-Mahmoudi, A.H.J.; Al-Nailey, K.G.C.; Ali, M.J.; Alfatlawi, M.A.A. & Al-Saeedi, T.A. 2019. Clinical, Parasitic and Histopathological study of Pigeons infested with Raillietina spp. in Al-Qadisiyah province, Iraq. International Journal of Drug Delivery Technology 9(2): 246-249.
Alvi, M.A.; Ohiolei, J.A.; Li, L.; Saqib, M.; Hussain, M.H. & Tayyab, M.H. 2020. In-house developed ELISA indicates high prevalence of anti-echinococcus granulosus IGG in sheep population-an update from Pakistan. Pathogens 9(11): 1-11.
Brik, K.; Hassouni, T.; Youssir, S.; Baroud, S.; Elkharrim, K. & Belghyti, D. 2018. Epidemiological study of Echinococcus granulosus in sheep in the Gharb plain (North-West of Morocco). Journal of Parasitic Diseases 42(4): 505-510.
Craig, P.; Mastin, A.; van Kesteren, F. & Boufana, B. 2015. Echinococcus granulosus: epidemiology and state-of-the-art of diagnostics in animals. Veterinary Parasitology 213(3-4): 132-148.
Hüttner, M.; Nakao, M.; Wassermann, T.; Siefert, L.; Boomker, J.D.; Dinkel, A. & Ito, A. 2008. Genetic characterization and phylogenetic position of Echinococcus felidis (Cestoda: Taeniidae) from the African lion. International Journal for Parasitology 38(7): 861-868.
Jin, Y.; Anvarov, K., Khajibaev, A.; Hong, S. & Hong, S.T. 2013. Serodiagnosis of echinococcosis by ELISA using cystic fluid from Uzbekistan sheep. The Korean Journal of Parasitology 51(3): 313.
Lymbery, A.J.; Jenkins, E.J.; Schurer, J.M. & Thompson, R.A. 2015. Echinococcus canadensis, E. borealis, and E. intermedius. What's in a name?. Trends in Parasitology 31(1): 23-29.
Nakao, M.; Lavikainen, A.; Yanagida, T. & Ito, A. 2013. Phylogenetic systematics of the genus Echinococcus (Cestoda: Taeniidae). International Journal for Parasitology 43: 1017-1029.
Romig, T.; Ebi, D. & Wassermann, M. 2015. Taxonomy and molecular epidemiology of Echinococcus granulosus sensu lato. Veterinary Parasitology 213(3-4): 76-84.
Scala, A.; Pipia, A.P.; Dore, F.; Sanna, G.; Tamponi, C.; Marrosu, R. & Varcasia, A. 2015. Epidemiological updates and economic losses due to Taenia hydatigena in sheep from Sardinia, Italy. Parasitology Research 114(8): 3137-3143.
Singh, B.B.; Sharma, R.; Gill, J.P.S. & Sharma, J.K. 2015. Prevalence and morphological characterisation of Cysticercus tenuicollis (Taenia hydatigena cysts) in sheep and goat from north India. Journal of Parasitic Diseases 39(1): 80-84.
Tianli, L.; Xifeng, W.; Zhenzhong, T.; Lixia, W.; Xingxing, Z.; Jun, Q. & Xuepeng, C. 2019. Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep. The Korean Journal of Parasitology 57(1): 61.
Valieva, Z.; Sarsembaeva, N.; Valdovska, A. & Ussenbayev, A.E. 2014. Impact of echinococcosis on quality of sheep meat in the South Eastern Kazakhstan. Asian-Australasian Journal of Animal Sciences 27(3): 391.