A note on the molecular systematics of the genus Paraschistura (Teleostei: Nemacheilidae) in Hari River Basin



 Nemacheilid loaches of the genus Paraschistura has been widely distributed from the Tigris drainage in Turkey, east throughout Iran and Pakistan to the Indus River and the Hari, Murghab and Helmand endorheic basins in Afghanistan, Iran, Pakistan and Turkmenistan. Herein, Paraschistura specimens from seven stations in Hari river basin were captured using hand-net and COI sequences of Paraschistura cristata and Paraschistura turcmenica were presented for all recognized species. Moreover; 119 sequences from ten species of Paraschistura in Genbank were added in order to assess of the phylogenetic position of the Paraschistura specimens and compare resulting tree with other studies. Molecular data obtained from Maximum likelihood (ML) tree and Bayesian tree (BI) were congruent and clearly indicate two main clades in phylogenetic tree and the genus Paraschistura in the Hari River basin, are considered as P. cristata and P. turcmenica.


Paraschistura cristata; Paraschistura turcmenica; Khorasan-Razavi Province- Hari River basin

Full Text:



Azimi, H.; Mousavi-sabet, H. & Eagderi, S. 2015a. Osteological characteristics of Paraschistura nielseni (Cypriniformes, Nemacheilidae). Iranian Journal of Ichthyol-ogy 2(3): 155-164.

Azimi, H.; Mousavi-Sabet, H.; Eagderi, S. & Vatandoust, S. 2015b. Osteological characteristics of Turkmenian stone loach, Paraschistura cristata (Cypriniformes: Nemacheilidae). International Journal of Aquatic Biology 3(5): 290-300.

Bănărescu, P. & Nalbant, T. 1995. A generical classification of Nemacheilinae with description of two new genera (Teleostei: Cypriniformes: Cobitidae). Travaux du Muséum d'Histoire Naturelle Grigore Antipa, Bucurešti 35: 429-496.

Bruford, M.W.; Hanotte, O.; Brookfield, J.F.; Burke, T. & Hoelzel, A. 1992. Single-locus and multi locus DNA fingerprint. In: Hoelzel, A.R. (ed.). Molecular Genetic Analysis of Populations: A Practical Approach. Oxford University Press, UK. pp. 225–270.

Coad, B.W. 2016. Freshwater Fishes of Iran. www.briancoad.com. Retrieved 06/05/2016.

Eschmeyer, W.N., & Fong, J.D. 2016. Catalog of fishes: genera, species, references. San Francisco, California Academy of Sciences. Esmaeili, H.R.; Masoudi, M.; Ebrahimi, M. & Elmi, A. 2016. Review of Aphanius farsicus: a critically endangered species (Teleostei: Cyprinodontidae) in Iran. Iranian Journal of Ichthyology 3(1): 1-18. Freyhof, J.; Sayyadzadeh, G.; Esmaeili, H.R. & Geiger, M. 2015. Review of the genus Paraschistura from Iran with description of six new species (Teleostei: Nemacheil-idae). Ichthyological Exploration of Fresh-waters 26(1): 1-48.

Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In: Nucleic Acids Symposium Series, pp. 95-98.

Hammer, Ø.; Harper D.A.T. & Ryan, P.D. 2001. PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica, 4: art. 4, 9 pp. http:// palaeo-electronica.org/2001_1/past/ issue1_01.htm.

Ivanova, N.V.; Dewaard, J.R. & Hebert, P.D. 2006. An inexpensive, automation‐friendly protocol for recovering high‐quality DNA. Molecular Ecology Notes 6 (4): 998-1002.

Jamali, H.; Patimar, R.; Farhadi, M.; Golzarianpour, K. & Daraei, V. 2014. Some aspects of the life history of Turcinoemacheilus hafezi (Teleostei: Nemacheilidae) from Beshar River, southwestern Iran. Iranian Journal of Ichthy- ology 1(1): 32-38.

Jamali, H.; Hasanpour-fattahi, A.; Abdollahi, D.; Patimar, R. & Keivany, Y. 2015. Some biological characteristics of Sefidrud loach, Oxynoemacheilus bergianus (Teleostei: Nemacheilidae), in Aras River, northwestern Caspian Sea basin. Iranian Journal of Ichthyology 2(1): 13-19.

Jamali, H.; Patimar, R.; Farhadi, M. & Daraei, V. 2016. Age, growth and reproduction of Paracobitis malapterura (Teleostei: Nemacheilidae) from Qom River, Iran. Iranian Journal of Ichthyology 3(1): 43-52. Jouladeh-Roudbar, A.; Eagderi, S. & Vatandoust, S. 2015. First record of Paraschistura alta (Nalbant and Bianco, 1998) from Eastern Iran and providing its COI barcode region sequences (Teleostei: Nemacheilidae). Iranian Journal of Ichthyology 2(4): 235-243.

Jouladeh-Roudbar, A.; Vatandoust, S.; Eagderi, S.; Jafari-Kenari, S. & Mousavi-Sabet, H. 2015. Freshwater fishes of Iran; an updated checklist. Aquaculture, Aquarium, Conservation & Legislation-International Journal of the Bioflux Society 8(6): 855-909.

Kottelat, M. 2012. Conspectus cobitidum: an inventory of the loaches of the world (Teleostei: Cypriniformes: Cobitoidei). The Raffles Bulletin of Zoology 26: 1-199.

Kottelat, M. & Freyhof, J. 2007. Handbook of European Freshwater Fishes. Kottelat, Cornol, Switzerland and Freyhof, Berlin, Germany. xiii+646p.

Lanfear, R.; Calcott, B.; Ho, S.Y. & Guindon, S. 2012. PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology & Evolution 29(6): 1695-1701.

Mousavi-Sabet, H. & Eagderi, S. 2015. Paraschistura delvarii spec. nov.–a new species of stone loach from the Persian Gulf basin, southern Iran (Teleostei: Nemacheilidae). Vertebrate Zoology 65(3): 297-303.

Mousavi Sabet, H.M.; Vatandoust, S.; Jouladeh-Roudbar, A. & Eagderi, S. 2015. Taxonomic Status of the Genus Paraschistura (Teleostei: Nemacheilidae) in the Hari River Basin, with Re-validation of P. turcomana. Journal of Applied Biological Sciences 9(3): 1-5.

Prokofiev, A.M. 2009. Problems of the classification and phylogeny of nemacheiline loaches of the group lacking the preethmoid I (Cypriniformes: Balitoridae: Nemacheilin-ae). Journal of Ichthyology 49(10): 874-898.

Prokofiev, A.M. 2010. Morphological classifica- tion of loaches (Nemacheilinae). Journal of Ichthyology 50(10): 827-913.

Ronquist, F.; Teslenko, M.; van der Mark, P.; Ayres, D.L.; Darling, A.; Höhna, S.; Larget, B.; Liu, L.; Suchard, M.A. & Huelsenbeck, J.P. 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61(3): 539-542.

Sayyadzadeh, G.; Eagderi, S. & Esmaeili, H.R. 2016. A new loach of the genus Oxynoemacheilus from the Tigris River drainage and its phylogenetic relationships among the nemacheilid fishes (Teleostei: Nemacheilidae) in the Middle East based on mtDNA COI sequences. Iranian Journal of Ichthyology 3(4): 236-250.

Stamatakis, A. 2006. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22 (21): 2688-2690.

Thompson, J.D.; Gibson, T.J.; Plewniak, F.; Jeanmougin, F. & Higgins, D.G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25 (24): 4876-4882.

Ward, R.D.; Hanner, R. & Hebert, P.D. 2009. The campaign to DNA barcode all fishes. Journal of Fish Biology 74(2): 329-356. Yazdani-Moghaddam, F.; Ghasemian, F.; Ghassemzadeh, F.; Khazaee, A.R.; Seifali, M. & Ghanbarifardi, M. 2015. The Fish Fauna of North and East Regions of Khorasan-e-Razavi Province, Iran. Iranian Journal of Animal Biosystematics 11(1): 91-100.


  • There are currently no refbacks.