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Abstract

The Caspian Kutum (Rutilus frisii, Nordmann, 1840) is an economically significant species among bony fish stocks in the southern basin of Caspian Sea. Nevertheless, information for transcriptome, the physiological differences and the regulatory mechanism of controlling for two strains remain unclear. To address this issue, we performed de novo assembly of liver transcriptome from six female R. frisii, three from each strain, using RNA sequencing (RNA-seq). After quality control, the de novo transcriptome assembly carried out with Trinity software and then 110,381 transcripts were generated. A total of 12,569 unigenes were grouped into three major functional categories (biological process, cellular components and molecular functions) and 62 sub-categories. A total of 9,627 transcripts were assigned at least one GO term. According to the public protein databases, we observed 21 unigenes related to reproduction by comparing the assembled unigenes and then 12849 differentially expressed genes (DEGs) were identified. We compared the histology of ovaries between the two strains to determine the degree of sexual maturation. The results of the present study provide a basis for future studies on the genetic differences between these two strains of R. frisii and their impact on population structure.

Keywords

Caspian Kutum Reproduction RNA-seq Transcriptome analysis

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The authors transfer the copyrights of their papers to the Iranian Society of Ichthyology. However, the information could be used in accordance with the Creative Commons licence (Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

How to Cite
FARASATI, S., KHOSHKHOLGH, M., & NAZARI, S. (2025). Transcriptome profiling of the liver among the spawning females of the Caspian Kutum (<i>Rutilus frisii</i>, Nordmann, 1840). Iranian Journal of Ichthyology, 12(3), 233–247. https://doi.org/10.22034/iji.v12i3.1109
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