Main Article Content
Abstract
The aim of this study was to investigate the effects of olive leaves extract on paw thickness, TNF-α, IL-6, RF, bilirubin, albumin and MDA in male albino rates with arthritis and compare it with Naproxen drug. A total of 12 mature male albino rates were divided into four groups, as 3 male rates for each group, and the experiment continued for 14 days. The animals were divided as follow: G1: treated as negative control group, injected with 0.1ml/ physiological normal saline (0.9% Nacl) in the first and third day of the experiment, G2: treated as positive control injected with 0.1 ml/ animal of formaldehyde (2%) in the first and third day of the experiment (Arthritis group), T1: as arthritis group, treated with olive leaf extract orally as 45mg/kg of B.W once daily and T2: as arthritis group, treated with Naproxen tablet orally as 100mg/ kg of B.W once daily. The results showed a significant increase in the paw thickness, TNF-α, IL-6, RF, bilirubin, albumin and MDA in the positive control compared with the negative control group and others groups. The results also showed a significant decrease in the paw thickness, TNF-α, IL-6RF, bilirubin, albumin and MDA in the T1 and T2. Also the results showed a significant decrease in the paw thickness, TNF-α, IL-6, RF, bilirubin, albumin and MDA in T2 group compared with T1. The present study showed that olive leaves extract attenuates Rheumatoid arthritis to be developed by reducing the levels of TNF-α, IL-6, RF, MDA, bilirubin and albumin and its extract was being more safety from Naproxen drug because the adverse effects of Naproxen drug.
Keywords
Antioxidant
Olive Leaves extract
Arthritis disease
Rat.
Article Details
License
The authors transfer the copyrights of their papers to the Iranian Society of Ichthyology. However, the information could be used in accordance with the Creative Commons licence (Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
How to Cite
ALETHARI, I. A. M., MOHAMMED, Z. I., & HAMMED, N. M. (2021). Antioxidant benefit of olive leaves extract on arthritis disease induced experimentally in rats. Iranian Journal of Ichthyology, 8, 194–199. Retrieved from http://www.ijichthyol.org/index.php/iji/article/view/010027
References
-
Ahmad-Qasem, M.H.; Barrajón-Catalán, E.; Micol, V.; Mulet, A. & García-Pérez, J.V. 2013. Influence of freezing and dehydration of olive leaves (var. Serrana) on extract composition and antioxidant potential. Food Research International 50: 189-96.
Benavente-Garcıa, O.; Castillo, J.; Lorente, J.; Ortuño, A.D.R.J. & Del Rio, J.A. 2000. Antioxidant activity of phenolics extracted from Olea europaea L. leaves. Food chemistry 68(4): 457-462.
Dekanski, D.; Selaković, V.; Piperski, V.; Radulović, Ž.; Korenić, A. & Radenović, L. 2011. Protective effect of olive leaf extract on hippocampal injury induced by transient global cerebral ischemia and reperfusion in Mongolian gerbils. Phytomedicine 18(13): 1137-1143.
Dekanski, D.; Selaković, V.; Piperski, V.; Radulović, Ž.; Korenić, A. & Radenović, L. 2011. Protective effect of olive leaf extract on hippocampal injury induced by transient global cerebral ischemia and reperfusion in Mongolian gerbils. Phytomedicine 18(13): 1137-1143.
Dimitrios, B. 2006. Sources of natural phenolic antioxidants. Trends in Food Science & Technology 17(9): 505-512.
Esmaeili-Mahani, S.; Rezaeezadeh-Roukerd, M.; Esmaeilpour, K.; Abbasnejad, M.; Rasoulian, B.; Sheibani, V. & Hajializadeh, Z. 2010. Olive (Olea europaea L.) leaf extract elicits antinociceptive activity, potentiates morphine analgesia and suppresses morphine hyperalgesia in rats. Journal of Ethnopharmacology 132(1): 200-205.
Hassen, I.; Casabianca, H. and Hosni, K. 2015. Biological activities of the natural antioxidant oleuropein: exceeding the expectation-a mini-review. Journal of Functional Foods 18: 926-940.
Hemmati, F. & Hashemi, Z. 2016. Evaluation of the predictive value of umbilical cord serum bilirubin level for the development of subsequent hyperbilirubinemia in term and late-preterm neonates. Iranian Journal of Neonatology 7(1):25-31.
Japon-Lujan, R.; Luque-Rodriguez, J.M. & Luque de Castro, M.D. 2006. Dynamic ultrasound-assisted extraction of oleuropein and related polyphenols from olive leaves. Journal of Chromatography A 1108(1): 76-82.
Kermanshah, Z.; Samadanifard, H.; Moghaddam, O.M. & Hejrati, A. 2020. Olive leaf and its various health-benefitting effects: a review study. Pakistan Journal of Medical & Health Sciences 14(2): 1301-1312.
Kore, K.J.; Shete, R.V. & Desai, N.V. 2011. Anti-arthritic activity of hydro alcoholic extract of Lawsonia innermis. International Journal of Drug Development & Research 3(4): 217-24.
Leech, N.L.; Barrett, K.C. & Morgan, G.A. 2011. IBMSPSS for intermediate statistics. 4th (ed). Taylor and Francis Group. LLC.USA.
Martín-García, A.I. & Molina-Alcaide, E. 2008. Effect of different drying procedures on the nutritive value of olive (Olea europaea var. europaea) leaves for ruminants. Animal Feed Science and Technology 142: 317-29.
Molina-Alcaide, E. & Yáñez-Ruiz, D.R. 2008. Potential use of olive by-products in ruminant feeding: A review. Animal Feed Science and Technology 147: 247-64.
Ray, N.B.; Lam, N.T.; Luc, R.; Bonvino, N.P. & Karagiannis, T.C. 2015. Cellular and molecular effects of bioactive phenolic compounds in olives and olive oil. In Olive and olive oil bioactive constituents (pp. 53-91). AOCS Press.
Rodkey, F.L. 1965. Direct spectrophotometric determination of albumin in human serum. Clinical Chemistry 11(4): 478-487.
Shabgah, A.G.; Qasim, M.T.; Mostafavi, S.M.; Zekiy, A.O.; Ezzatifar, F.; Ahmadi, M. & Navashenaq, J.G. 2021. CXC chemokine ligand 16: a Swiss army knife chemokine in cancer. Expert Reviews in Molecular Medicine 23.
Singh, I.; Mok, M.; Christensen, A.M.; Turner, A.H. & Hawley, J.A. 2008. The effects of polyphenols in olive leaves on platelet function. Nutrition, Metabolism and Cardiovascular Diseases 18(2):127-132.
Somova, L.I.; Shode, F.O.; Ramnanan. P. & Nadar, A. 2003. Antihypertensive, antiatherosclerotic and antioxidant activity of triterpenoids isolated from Olea europaea, subspecies Africana leaves. Journal of Ethnopharmacol 84: 299-305.
Soni, M.G.; Burdock, G.A.; Christian, M.S.; Bitler, C.M. & Crea, R. 2006. Safety assessment of aqueous olive pulp extract as an antioxidant or antimicrobial agent in foods. Food and Chemical Toxicology 44(7): 903-915.
Tahmasebi, S.; Qasim, M.T.; Krivenkova, M.V.; Zekiy, A.O.; Thangavelu, L.; Aravindhan, S. & Roshangar, L. 2021. The effects of oxygen–ozone therapy on regulatory T‐cell responses in multiple sclerosis patients. Cell Biology International.
Visioli, F.; Poli, A. & Gall, C. 2002. Antioxidant and other biological activities of phenols from olives and olive oil. Medicinal Research Reviews 22(1): 65-75.
Zhang, Y., Wen, M., Zhou, P., Tian, M., Zhou, J. and Zhang, L., 2020. Analysis of chemical composition in Chinese olive leaf tea by UHPLC-DAD-Q-TOF-MS/MS and GC–MS and its lipid-lowering effects on the obese mice induced by high-fat diet. Food Research International, 128, p.108785.
Zhang, Y.; Wen, M.; Zhou, P.; Tian, M.; Zhou, J. & Zhang, L. 2020. Analysis of chemical composition in Chinese olive leaf tea by UHPLC-DAD-Q-TOF-MS/MS and GC–MS and its lipid-lowering effects on the obese mice induced by high-fat diet. Food Research International 128: 108785.
References
Ahmad-Qasem, M.H.; Barrajón-Catalán, E.; Micol, V.; Mulet, A. & García-Pérez, J.V. 2013. Influence of freezing and dehydration of olive leaves (var. Serrana) on extract composition and antioxidant potential. Food Research International 50: 189-96.
Benavente-Garcıa, O.; Castillo, J.; Lorente, J.; Ortuño, A.D.R.J. & Del Rio, J.A. 2000. Antioxidant activity of phenolics extracted from Olea europaea L. leaves. Food chemistry 68(4): 457-462.
Dekanski, D.; Selaković, V.; Piperski, V.; Radulović, Ž.; Korenić, A. & Radenović, L. 2011. Protective effect of olive leaf extract on hippocampal injury induced by transient global cerebral ischemia and reperfusion in Mongolian gerbils. Phytomedicine 18(13): 1137-1143.
Dekanski, D.; Selaković, V.; Piperski, V.; Radulović, Ž.; Korenić, A. & Radenović, L. 2011. Protective effect of olive leaf extract on hippocampal injury induced by transient global cerebral ischemia and reperfusion in Mongolian gerbils. Phytomedicine 18(13): 1137-1143.
Dimitrios, B. 2006. Sources of natural phenolic antioxidants. Trends in Food Science & Technology 17(9): 505-512.
Esmaeili-Mahani, S.; Rezaeezadeh-Roukerd, M.; Esmaeilpour, K.; Abbasnejad, M.; Rasoulian, B.; Sheibani, V. & Hajializadeh, Z. 2010. Olive (Olea europaea L.) leaf extract elicits antinociceptive activity, potentiates morphine analgesia and suppresses morphine hyperalgesia in rats. Journal of Ethnopharmacology 132(1): 200-205.
Hassen, I.; Casabianca, H. and Hosni, K. 2015. Biological activities of the natural antioxidant oleuropein: exceeding the expectation-a mini-review. Journal of Functional Foods 18: 926-940.
Hemmati, F. & Hashemi, Z. 2016. Evaluation of the predictive value of umbilical cord serum bilirubin level for the development of subsequent hyperbilirubinemia in term and late-preterm neonates. Iranian Journal of Neonatology 7(1):25-31.
Japon-Lujan, R.; Luque-Rodriguez, J.M. & Luque de Castro, M.D. 2006. Dynamic ultrasound-assisted extraction of oleuropein and related polyphenols from olive leaves. Journal of Chromatography A 1108(1): 76-82.
Kermanshah, Z.; Samadanifard, H.; Moghaddam, O.M. & Hejrati, A. 2020. Olive leaf and its various health-benefitting effects: a review study. Pakistan Journal of Medical & Health Sciences 14(2): 1301-1312.
Kore, K.J.; Shete, R.V. & Desai, N.V. 2011. Anti-arthritic activity of hydro alcoholic extract of Lawsonia innermis. International Journal of Drug Development & Research 3(4): 217-24.
Leech, N.L.; Barrett, K.C. & Morgan, G.A. 2011. IBMSPSS for intermediate statistics. 4th (ed). Taylor and Francis Group. LLC.USA.
Martín-García, A.I. & Molina-Alcaide, E. 2008. Effect of different drying procedures on the nutritive value of olive (Olea europaea var. europaea) leaves for ruminants. Animal Feed Science and Technology 142: 317-29.
Molina-Alcaide, E. & Yáñez-Ruiz, D.R. 2008. Potential use of olive by-products in ruminant feeding: A review. Animal Feed Science and Technology 147: 247-64.
Ray, N.B.; Lam, N.T.; Luc, R.; Bonvino, N.P. & Karagiannis, T.C. 2015. Cellular and molecular effects of bioactive phenolic compounds in olives and olive oil. In Olive and olive oil bioactive constituents (pp. 53-91). AOCS Press.
Rodkey, F.L. 1965. Direct spectrophotometric determination of albumin in human serum. Clinical Chemistry 11(4): 478-487.
Shabgah, A.G.; Qasim, M.T.; Mostafavi, S.M.; Zekiy, A.O.; Ezzatifar, F.; Ahmadi, M. & Navashenaq, J.G. 2021. CXC chemokine ligand 16: a Swiss army knife chemokine in cancer. Expert Reviews in Molecular Medicine 23.
Singh, I.; Mok, M.; Christensen, A.M.; Turner, A.H. & Hawley, J.A. 2008. The effects of polyphenols in olive leaves on platelet function. Nutrition, Metabolism and Cardiovascular Diseases 18(2):127-132.
Somova, L.I.; Shode, F.O.; Ramnanan. P. & Nadar, A. 2003. Antihypertensive, antiatherosclerotic and antioxidant activity of triterpenoids isolated from Olea europaea, subspecies Africana leaves. Journal of Ethnopharmacol 84: 299-305.
Soni, M.G.; Burdock, G.A.; Christian, M.S.; Bitler, C.M. & Crea, R. 2006. Safety assessment of aqueous olive pulp extract as an antioxidant or antimicrobial agent in foods. Food and Chemical Toxicology 44(7): 903-915.
Tahmasebi, S.; Qasim, M.T.; Krivenkova, M.V.; Zekiy, A.O.; Thangavelu, L.; Aravindhan, S. & Roshangar, L. 2021. The effects of oxygen–ozone therapy on regulatory T‐cell responses in multiple sclerosis patients. Cell Biology International.
Visioli, F.; Poli, A. & Gall, C. 2002. Antioxidant and other biological activities of phenols from olives and olive oil. Medicinal Research Reviews 22(1): 65-75.
Zhang, Y., Wen, M., Zhou, P., Tian, M., Zhou, J. and Zhang, L., 2020. Analysis of chemical composition in Chinese olive leaf tea by UHPLC-DAD-Q-TOF-MS/MS and GC–MS and its lipid-lowering effects on the obese mice induced by high-fat diet. Food Research International, 128, p.108785.
Zhang, Y.; Wen, M.; Zhou, P.; Tian, M.; Zhou, J. & Zhang, L. 2020. Analysis of chemical composition in Chinese olive leaf tea by UHPLC-DAD-Q-TOF-MS/MS and GC–MS and its lipid-lowering effects on the obese mice induced by high-fat diet. Food Research International 128: 108785.
Benavente-Garcıa, O.; Castillo, J.; Lorente, J.; Ortuño, A.D.R.J. & Del Rio, J.A. 2000. Antioxidant activity of phenolics extracted from Olea europaea L. leaves. Food chemistry 68(4): 457-462.
Dekanski, D.; Selaković, V.; Piperski, V.; Radulović, Ž.; Korenić, A. & Radenović, L. 2011. Protective effect of olive leaf extract on hippocampal injury induced by transient global cerebral ischemia and reperfusion in Mongolian gerbils. Phytomedicine 18(13): 1137-1143.
Dekanski, D.; Selaković, V.; Piperski, V.; Radulović, Ž.; Korenić, A. & Radenović, L. 2011. Protective effect of olive leaf extract on hippocampal injury induced by transient global cerebral ischemia and reperfusion in Mongolian gerbils. Phytomedicine 18(13): 1137-1143.
Dimitrios, B. 2006. Sources of natural phenolic antioxidants. Trends in Food Science & Technology 17(9): 505-512.
Esmaeili-Mahani, S.; Rezaeezadeh-Roukerd, M.; Esmaeilpour, K.; Abbasnejad, M.; Rasoulian, B.; Sheibani, V. & Hajializadeh, Z. 2010. Olive (Olea europaea L.) leaf extract elicits antinociceptive activity, potentiates morphine analgesia and suppresses morphine hyperalgesia in rats. Journal of Ethnopharmacology 132(1): 200-205.
Hassen, I.; Casabianca, H. and Hosni, K. 2015. Biological activities of the natural antioxidant oleuropein: exceeding the expectation-a mini-review. Journal of Functional Foods 18: 926-940.
Hemmati, F. & Hashemi, Z. 2016. Evaluation of the predictive value of umbilical cord serum bilirubin level for the development of subsequent hyperbilirubinemia in term and late-preterm neonates. Iranian Journal of Neonatology 7(1):25-31.
Japon-Lujan, R.; Luque-Rodriguez, J.M. & Luque de Castro, M.D. 2006. Dynamic ultrasound-assisted extraction of oleuropein and related polyphenols from olive leaves. Journal of Chromatography A 1108(1): 76-82.
Kermanshah, Z.; Samadanifard, H.; Moghaddam, O.M. & Hejrati, A. 2020. Olive leaf and its various health-benefitting effects: a review study. Pakistan Journal of Medical & Health Sciences 14(2): 1301-1312.
Kore, K.J.; Shete, R.V. & Desai, N.V. 2011. Anti-arthritic activity of hydro alcoholic extract of Lawsonia innermis. International Journal of Drug Development & Research 3(4): 217-24.
Leech, N.L.; Barrett, K.C. & Morgan, G.A. 2011. IBMSPSS for intermediate statistics. 4th (ed). Taylor and Francis Group. LLC.USA.
Martín-García, A.I. & Molina-Alcaide, E. 2008. Effect of different drying procedures on the nutritive value of olive (Olea europaea var. europaea) leaves for ruminants. Animal Feed Science and Technology 142: 317-29.
Molina-Alcaide, E. & Yáñez-Ruiz, D.R. 2008. Potential use of olive by-products in ruminant feeding: A review. Animal Feed Science and Technology 147: 247-64.
Ray, N.B.; Lam, N.T.; Luc, R.; Bonvino, N.P. & Karagiannis, T.C. 2015. Cellular and molecular effects of bioactive phenolic compounds in olives and olive oil. In Olive and olive oil bioactive constituents (pp. 53-91). AOCS Press.
Rodkey, F.L. 1965. Direct spectrophotometric determination of albumin in human serum. Clinical Chemistry 11(4): 478-487.
Shabgah, A.G.; Qasim, M.T.; Mostafavi, S.M.; Zekiy, A.O.; Ezzatifar, F.; Ahmadi, M. & Navashenaq, J.G. 2021. CXC chemokine ligand 16: a Swiss army knife chemokine in cancer. Expert Reviews in Molecular Medicine 23.
Singh, I.; Mok, M.; Christensen, A.M.; Turner, A.H. & Hawley, J.A. 2008. The effects of polyphenols in olive leaves on platelet function. Nutrition, Metabolism and Cardiovascular Diseases 18(2):127-132.
Somova, L.I.; Shode, F.O.; Ramnanan. P. & Nadar, A. 2003. Antihypertensive, antiatherosclerotic and antioxidant activity of triterpenoids isolated from Olea europaea, subspecies Africana leaves. Journal of Ethnopharmacol 84: 299-305.
Soni, M.G.; Burdock, G.A.; Christian, M.S.; Bitler, C.M. & Crea, R. 2006. Safety assessment of aqueous olive pulp extract as an antioxidant or antimicrobial agent in foods. Food and Chemical Toxicology 44(7): 903-915.
Tahmasebi, S.; Qasim, M.T.; Krivenkova, M.V.; Zekiy, A.O.; Thangavelu, L.; Aravindhan, S. & Roshangar, L. 2021. The effects of oxygen–ozone therapy on regulatory T‐cell responses in multiple sclerosis patients. Cell Biology International.
Visioli, F.; Poli, A. & Gall, C. 2002. Antioxidant and other biological activities of phenols from olives and olive oil. Medicinal Research Reviews 22(1): 65-75.
Zhang, Y., Wen, M., Zhou, P., Tian, M., Zhou, J. and Zhang, L., 2020. Analysis of chemical composition in Chinese olive leaf tea by UHPLC-DAD-Q-TOF-MS/MS and GC–MS and its lipid-lowering effects on the obese mice induced by high-fat diet. Food Research International, 128, p.108785.
Zhang, Y.; Wen, M.; Zhou, P.; Tian, M.; Zhou, J. & Zhang, L. 2020. Analysis of chemical composition in Chinese olive leaf tea by UHPLC-DAD-Q-TOF-MS/MS and GC–MS and its lipid-lowering effects on the obese mice induced by high-fat diet. Food Research International 128: 108785.