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Abstract

Siamese fighting fish (Betta splendens) is originated from Southeast Asia and one of Indonesia’s most profitable exports commodity. However, scientific studies on Siamese fighting fish chromosomes remain limited. Chromosomal information is very useful for disclosing the diversity and genetic relationships of animals and provides clues to the most appropriate conservation measures for a species. Moreover, affordable chromosome visualization procedures are also a main concern for animal research. This research aims to determine the optimal chromosome preparation time, the best method for observing prometaphase, and the chromosomal characteristics of different types of betta fish based on their diploid numbers. The results showed that the optimal time for chromosome preparation is between 07:00 am and 12:00 pm and that the best method for observing chromosomes is the splash method from Kligerman and Bloom modified by soaking samples in colchicine for 11-12 h. The chromosome numbers of Giant, Plakat, Crown tail, and Halfmoon bettas are identical, i.e., 2n=42.

Keywords

Siamese fighting fish Chromosomes number Cytogenetic Mitosis time

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The authors transfer the copyrights of their papers to the Iranian Society of Ichthyology. However, the information could be used in accordance with the Creative Commons licence (Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

How to Cite
DARYONO, B. S., KOENTJANA, J. P., & PERDAMAIAN, A. B. I. (2023). Chromosomes number and mitosis time of Siamese fighting fish (Betta splendens Regan, 1910). Iranian Journal of Ichthyology, 9(3), 158–164. https://doi.org/10.22034/iji.v9i3.845
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References

  1. Abu-Almaaty, A.; Hassan, M.; Bahgat, I.M. & Suleiman, M.E. 2017. Inter Simple Sequence Repeat (ISSR) and cytogenetic analysis of three fish species of Family Osphronemidae. Egyptian Journal of Aquatic Biology and Fisheries 21(2): 1-15.
  2. Amini, F. & Sokoot, A.S. 2013. Karyotyping and chromosomal Ag-NOR banding of siamese fighting fish (Betta splendens). Journal of Veterinary Research 68(3): 249-255
  3. Eisenreich, B.R. & Szalda-Petree, A. 2015. Behavioral effects of fluoxetine on aggression and associative learning in Siamese fighting fish (Betta splendens). Behavioural Processes 121: 37-42.
  4. Eisenreich, B.R.; Greene, S. & Szalda-Petree, A. 2017. Of fish and mirrors: Fluoxetine disrupts aggression and learning for social rewards. Physiology & Behavior 173: 258-262.
  5. Fan, G.; Chan, J.; Ma, K.; Yang, B.; Zhang, H.; Yang, X.; Shi, C.; Chun-Hin Law, H.; Ren, Z.; Xu, Q.; Liu, Q.; Wang, J.; Chen, W.; Shao, L.; Gonçalves, D.; Ramos, A.; Cardoso, S.D.; Guo, M.; Cai, J.; Xu, X.; Wang, J.; Yang, H.; Liu, X. & Wang, Y. 2018. Chromosome-level reference genome of the Siamese fighting fish Betta splendens, a model species for the study of aggression. Gigascience 7(11): giy087.
  6. Foresti, F.; Oliveira, C. & Foresti de Almeida-Toledo, L. 1993. A method for chromosome preparations from large fish specimens using in vitro short-term treatment with colchicine. Experientia 49: 810-813.
  7. Forsatkar, M.N.; Nematollahi, M.A.; Amiri, B.M. & Huang, W.B. 2014. Fluoxetine inhibits aggressive behavior during parental care in male fighting fish (Betta splendens Regan). Ecotoxicology 23(9): 1794-802.
  8. Hedayati Rad, M., Nematollahi, M.A., Forsatkar, M.N. & Brown, C. 2017. Prozac impacts lateralization of aggression in male Siamese fighting fish. Ecotoxicology and Environmental Safety 140: 84-88.
  9. Hu, F.; Fan, J.; Qin, Q.; Huo, Y.; Wang, Y.; Wu, C.; Liu, Q.; Li, W.; Chen, X.; Cao, L.; Tao, M.; Wang, S.; Zhao, R.; Luo, K. & Liu, S. 2019. The sterility of allotriploid fish and fertility of female autotriploid fish. Frontiers in Genetics 10: 377.
  10. Karami, A.; Eghtesadi Araghi, P.; Syed, M.A. & Wilson, S.P. 2015. Chromosome preparation in fish: effects of fish species and larval age. International Aquatic Research 7: 201-210.
  11. Kligerman, A.D. & Bloom, S.E. 1977. Rapid chromosome preparations from solid tissues of fishes. Journal of Fishery Research Board of Canada 34: 266-269.
  12. Lichak, M.R.; Barber, J.R.; Kwon, Y.M.; Francis, K.X. & Bendesky, A. 2022. Care and use of Siamese fighting fish (Betta Splendens) for Research. Comparative Medicine 72(3):169-180.
  13. Mandal, S.C.; Sahu, N.P.; Kohli, M.P.S.; Das, P.; Gupta, S.K. & Munilkumar, S. 2010. Replacement of live feed by formulated feed: effect on the growth and spawning performance of Siamese fighting fish (Betta splendens Regan, 1910). Aquaculture Research 41: 1707-1716.
  14. Norazmi-Lokman, N.H.; Baderi, A.A.; Zabidi, Z.M. & Diana, A.W. 2020. Effects of different feeding frequency on Siamese fighting fish (Betta splenden) and Guppy (Poecilia reticulata) Juveniles: Data on growth performance and survival rate. Data Brief 32: 106046.
  15. Prost, S.; Petersen, M.; Grethlein, M.; Hahn, S.J.; Kuschik-Maczollek, N.; Olesiuk, M.E.; Reschke, J.O.; Schmey, T.E.; Zimmer, C.; Gupta, D.K.; Schell, T.; Coimbra, R.; De Raad, J.; Lammers, F.; Winter. S. & Janke, A. 2020. Improving the chromosome-level genome assembly of the Siamese fighting fish (Betta splendens) in a University Master's Course. G3: Genes, Genomes, Genetics 10(7): 2179-2183.
  16. Purivirojkul, W. 2012. Potential application of extracts from Indian almond (Terminalia catappa Linn.) leaves in Siamese fighting fish (Betta splendens Regan) culture. Communications in Agricultural and Applied Biological Science 77(4): 439-48.
  17. Ramos, A. & Gonçalves, D. 2019. Artificial selection for male winners in the Siamese fighting fish Betta splendens correlates with high female aggression. Frontier in Zoology 16(1): 34.
  18. Ratanatham, S. & Patinawin, S. 1978. Cytogenetic studies of Siamese fighting fish (Betta splendens Regan). Journal of the science society of Thailand 5: 17-26.
  19. Saekhow, S.; Thongprajukaew, K.; Phromkunthong, W. &Sae-Khoo, H. 2018. Minimal water volume for intensively producing male Siamese fighting fish (Betta splendens Regan, 1910). Fish Physiology and Biochemistry 44(4): 1075-1085.
  20. Selezniow, F.; Fopp-Bayat, G.D.; Jankun, M.; Krejszeff, S. & Mamcarz, A. 2008. Note on the karyotype and nor location of Siamese fighting fish Betta splendens (Perciformes, Osphronemidae). Caryologia 61(4): 349-353.
  21. Sipaúba-Tavares, L.H.; Appoloni, A.M.; Fernandes, J.B.K. & Millan, R.N. 2016. Feed of Siamese fighting fish, Betta splendens (Regan, 1910) in open pond: live and formulated diets. Brazilian Journal of Biology 76(2): 292-299.
  22. Song, Y.N.; Xiao, G.B. & Li, J.T. 2016. Complete mitochondrial genome of the Siamese fighting fish (Betta splendens). Mitochondrial DNA Part A 27(6): 4580-4581.
  23. Tjio, J.H. & Wang, J. 1960. Chromosome preparations of bone marrow cells without prior in vitro culture or in vivo colchicine administration. Stain Technology 37(1): 17-20.
  24. Vu, T.D.; Iwasaki, Y.; Shigenobu, S.; Maruko, A.; Oshima, K.; Iioka, E.; Huang, C.L.; Abe, T.; Tamaki, S.; Lin, Y.W.; Chen, C.K.; Lu, M.Y.; Hojo, M.; Wang, H.V.; Tzeng, S.F.; Huang, H.J.; Kanai, A.; Gojobori, T.; Chiang, T.Y.; Sun, H.S.; Li, W.H. & Okada, N. 2020. Behavioral and brain- transcriptomic synchronization between the two opponents of a fighting pair of the fish Betta splendens. PLOS Genetics 16(6): e1008831.
  25. Zhang, W.; Wang, H.; Brandt, D.Y.C.; Hu, B.; Sheng, J.; Wang, M.; Luo, H.; Li, Y.; Guo, S.; Sheng, B.; Zeng, Q.; Peng, K.; Zhao, D.; Jian, S.; Wu, D.; Wang, J.; Zhao, G.; Ren, J.; Shi, W.; van Esch, J.H.M.; Klingunga, S.; Nielsen, R. & Hong, Y. 2022. The genetic architecture of phenotypic diversity in the Betta fish (Betta splendens). Science Advance 8(38): eabm4955.