Main Article Content
Abstract
Oxynoemacheilus parvinae, new species, from the tributaries of the Iranian Sirvan River drainage, belongs to a group of Oxynoemacheilus having a suborbital groove in males and elongated body. It is distinguished from the other species of this group in the Tigris River drainage by a combination of the following characters: 8½-9½ branched dorsal-fin rays, large to medium dark-brown spots especially on the post-dorsal part, emarginated caudal fin, angular shape of bony capsule of swim bladder with developed posterior process, longer head and shallower body. It is also diagnosed from its nearest species (O. bergianus and O. longipinnis) by two fixed, diagnostic nucleotide substitutions in the mtDNA COI barcode region, and a K2P nearest-neighbour distance of 1.4% to O. longipinnis. Maximum Likelihood and Bayesian phylogeny reconstructed based on COI barcode region place the sequenced nemacheilid fish into a monophyletic clade which show between 1.4 % (O. parvinae vs. O. longipinnis) and 16.3 % (O. longipinnis vs. O. merga) K2P sequence divergence.
Keywords
Freshwater fish
Middle East
Nemacheilid fish
DNA barcoding.
Article Details
License
The authors transfer the copyrights of their papers to the Iranian Society of Ichthyology. However, the information could be used in accordance with the Creative Commons licence (Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
How to Cite
SAYYADZADEH, G., EAGDERI, S., & ESMAEILI, H. R. (2016). A new loach of the genus Oxynoemacheilus from the Tigris River drainage and its phylogenetic relationships among the nemacheilid fishes (Teleostei: Nemacheilidae) in the Middle East based on mtDNA COI sequences. Iranian Journal of Ichthyology, 3(4), 236–250. https://doi.org/10.22034/iji.v3i4.205
References
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Bahrami Kamangar, B.; Prokofiev, A.M.; Ghaderi, E. & Nalbant, T.T. 2014. Stone loaches of Choman River system, Kurdistan, Iran (Teleostei: Cyprini-formes: Nemacheilidae). Zootaxa 3755 (1): 033-061.
Eschmeyer, W.N. & Fong, J. 2016. Catalog of fishes: Species by Family/Subfamily. (http://research archive.calacademy.org/research/ichthyology/catalog/SpeciesByFamily.asp Electronic version accessed 12 August 2016.
Esmaeili, H.R.; Coad, B.W.; Gholamifard, A.; Nazari, N. & Teimory, A. 2010. Annotated checklist of the freshwater fishes of Iran. Zoosystematica Rossica 19: 361-386.
Freyhof, J.; Erk'akan, F.; Özeren, C. & Perdices, A.J. 2011. An overview of the western Palaearctic loach genus Oxynoemacheilus (Teleostei: Nemacheil-idae). Ichthyological Exploration of Freshwaters 22: 301-312.
Freyhof, J. 2016. Oxynoemacheilus karunensis, a new species from the Persian Gulf basin (Teleostei: Nemacheilidae). Zootaxa 4175: 94-100.
Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95-98.
Higgins, D.G. & Sharp, P.M. 1988. CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene 73: 237-244.
Huelsenbeck, J.P. & Ranala, B. 2004. Frequentist properties of Bayesian posterior probabilities of phylogenetic trees under simple and complex substitution models. Systematic Biology 53: 904-913.
Ivanova, N.V.; Zemlak, T.S.; Hanner, R.H. & Hebert, P.D.N. 2007. Universal primer cocktails for fish DNA barcoding. Molecular Ecology Notes 7: 544-548.
Jouladeh-Roudbar, A.; Vatandoust, S.; Eagderi, S.; Jafari-Kenari, S. & Mousavi-Sabet, H. 2015. Freshwater fishes of Iran; an updated checklist. Aquaculture, Aquarium, Conservation and Legislation, International Journal of the Bioflux 8: 855-909.
Jouladeh-Roudbar, A.; Eagderi, S. & Hosseinpour, T. 2016. Oxynoemacheilus freyhofi, a new nemacheilid species (Teleostei, Nemacheilidae) from the Tigris basin, Iran. FishTaxa 1(2): 94-107.
Kottelat, M. & Freyhof, J. 2007. Handbook of European Freshwater Fishes. Kottelat, Cornol and Freyhof, Berlin. 646 p.
Posada, D. & Crandall, K.A. 1998. MODELTEST: testing the model of DNA substitution. Bioinformatics 14: 817-818.
Ronquist, F. & Huelsenbeck, J.P. 2003. MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572-1574.
Sayyadzadeh, G.; Esmaeili, H.R; Eagderi, S.; Jouladeh-Roudbar, A.; Masoudi, M. & Vatandoust, S. 2016. Re-description and molecular systematics of Oxynoemacheilus longipinnis from the Persian Gulf basin (Teleostei, Nemacheilidae). Zookeys under review.
Stamatakis, A. 2006. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688-2690.
Tamura, K.; Peterson, D.; Peterson, N.; Stecher, G.; Nei, M. & Kumar, S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28: 2731–2739.
Toussaint, E.F.; Beutel, R.G.; Morinière, J.; Jia, F.; Xu, S.; Michat, M.C.; Zhou, X.; Bilton, D.T.; Ribera, I.; Hájek, J. & Balke, M. 2015. Molecular phylogeny of the highly disjunct cliff water beetles from South Africa and China (Coleoptera: Aspidytidae). Zoological Journal of the Linnean Society 176: 537-546.
References
Bahrami Kamangar, B.; Prokofiev, A.M.; Ghaderi, E. & Nalbant, T.T. 2014. Stone loaches of Choman River system, Kurdistan, Iran (Teleostei: Cyprini-formes: Nemacheilidae). Zootaxa 3755 (1): 033-061.
Eschmeyer, W.N. & Fong, J. 2016. Catalog of fishes: Species by Family/Subfamily. (http://research archive.calacademy.org/research/ichthyology/catalog/SpeciesByFamily.asp Electronic version accessed 12 August 2016.
Esmaeili, H.R.; Coad, B.W.; Gholamifard, A.; Nazari, N. & Teimory, A. 2010. Annotated checklist of the freshwater fishes of Iran. Zoosystematica Rossica 19: 361-386.
Freyhof, J.; Erk'akan, F.; Özeren, C. & Perdices, A.J. 2011. An overview of the western Palaearctic loach genus Oxynoemacheilus (Teleostei: Nemacheil-idae). Ichthyological Exploration of Freshwaters 22: 301-312.
Freyhof, J. 2016. Oxynoemacheilus karunensis, a new species from the Persian Gulf basin (Teleostei: Nemacheilidae). Zootaxa 4175: 94-100.
Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95-98.
Higgins, D.G. & Sharp, P.M. 1988. CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene 73: 237-244.
Huelsenbeck, J.P. & Ranala, B. 2004. Frequentist properties of Bayesian posterior probabilities of phylogenetic trees under simple and complex substitution models. Systematic Biology 53: 904-913.
Ivanova, N.V.; Zemlak, T.S.; Hanner, R.H. & Hebert, P.D.N. 2007. Universal primer cocktails for fish DNA barcoding. Molecular Ecology Notes 7: 544-548.
Jouladeh-Roudbar, A.; Vatandoust, S.; Eagderi, S.; Jafari-Kenari, S. & Mousavi-Sabet, H. 2015. Freshwater fishes of Iran; an updated checklist. Aquaculture, Aquarium, Conservation and Legislation, International Journal of the Bioflux 8: 855-909.
Jouladeh-Roudbar, A.; Eagderi, S. & Hosseinpour, T. 2016. Oxynoemacheilus freyhofi, a new nemacheilid species (Teleostei, Nemacheilidae) from the Tigris basin, Iran. FishTaxa 1(2): 94-107.
Kottelat, M. & Freyhof, J. 2007. Handbook of European Freshwater Fishes. Kottelat, Cornol and Freyhof, Berlin. 646 p.
Posada, D. & Crandall, K.A. 1998. MODELTEST: testing the model of DNA substitution. Bioinformatics 14: 817-818.
Ronquist, F. & Huelsenbeck, J.P. 2003. MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572-1574.
Sayyadzadeh, G.; Esmaeili, H.R; Eagderi, S.; Jouladeh-Roudbar, A.; Masoudi, M. & Vatandoust, S. 2016. Re-description and molecular systematics of Oxynoemacheilus longipinnis from the Persian Gulf basin (Teleostei, Nemacheilidae). Zookeys under review.
Stamatakis, A. 2006. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688-2690.
Tamura, K.; Peterson, D.; Peterson, N.; Stecher, G.; Nei, M. & Kumar, S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28: 2731–2739.
Toussaint, E.F.; Beutel, R.G.; Morinière, J.; Jia, F.; Xu, S.; Michat, M.C.; Zhou, X.; Bilton, D.T.; Ribera, I.; Hájek, J. & Balke, M. 2015. Molecular phylogeny of the highly disjunct cliff water beetles from South Africa and China (Coleoptera: Aspidytidae). Zoological Journal of the Linnean Society 176: 537-546.
Eschmeyer, W.N. & Fong, J. 2016. Catalog of fishes: Species by Family/Subfamily. (http://research archive.calacademy.org/research/ichthyology/catalog/SpeciesByFamily.asp Electronic version accessed 12 August 2016.
Esmaeili, H.R.; Coad, B.W.; Gholamifard, A.; Nazari, N. & Teimory, A. 2010. Annotated checklist of the freshwater fishes of Iran. Zoosystematica Rossica 19: 361-386.
Freyhof, J.; Erk'akan, F.; Özeren, C. & Perdices, A.J. 2011. An overview of the western Palaearctic loach genus Oxynoemacheilus (Teleostei: Nemacheil-idae). Ichthyological Exploration of Freshwaters 22: 301-312.
Freyhof, J. 2016. Oxynoemacheilus karunensis, a new species from the Persian Gulf basin (Teleostei: Nemacheilidae). Zootaxa 4175: 94-100.
Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95-98.
Higgins, D.G. & Sharp, P.M. 1988. CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene 73: 237-244.
Huelsenbeck, J.P. & Ranala, B. 2004. Frequentist properties of Bayesian posterior probabilities of phylogenetic trees under simple and complex substitution models. Systematic Biology 53: 904-913.
Ivanova, N.V.; Zemlak, T.S.; Hanner, R.H. & Hebert, P.D.N. 2007. Universal primer cocktails for fish DNA barcoding. Molecular Ecology Notes 7: 544-548.
Jouladeh-Roudbar, A.; Vatandoust, S.; Eagderi, S.; Jafari-Kenari, S. & Mousavi-Sabet, H. 2015. Freshwater fishes of Iran; an updated checklist. Aquaculture, Aquarium, Conservation and Legislation, International Journal of the Bioflux 8: 855-909.
Jouladeh-Roudbar, A.; Eagderi, S. & Hosseinpour, T. 2016. Oxynoemacheilus freyhofi, a new nemacheilid species (Teleostei, Nemacheilidae) from the Tigris basin, Iran. FishTaxa 1(2): 94-107.
Kottelat, M. & Freyhof, J. 2007. Handbook of European Freshwater Fishes. Kottelat, Cornol and Freyhof, Berlin. 646 p.
Posada, D. & Crandall, K.A. 1998. MODELTEST: testing the model of DNA substitution. Bioinformatics 14: 817-818.
Ronquist, F. & Huelsenbeck, J.P. 2003. MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572-1574.
Sayyadzadeh, G.; Esmaeili, H.R; Eagderi, S.; Jouladeh-Roudbar, A.; Masoudi, M. & Vatandoust, S. 2016. Re-description and molecular systematics of Oxynoemacheilus longipinnis from the Persian Gulf basin (Teleostei, Nemacheilidae). Zookeys under review.
Stamatakis, A. 2006. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688-2690.
Tamura, K.; Peterson, D.; Peterson, N.; Stecher, G.; Nei, M. & Kumar, S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28: 2731–2739.
Toussaint, E.F.; Beutel, R.G.; Morinière, J.; Jia, F.; Xu, S.; Michat, M.C.; Zhou, X.; Bilton, D.T.; Ribera, I.; Hájek, J. & Balke, M. 2015. Molecular phylogeny of the highly disjunct cliff water beetles from South Africa and China (Coleoptera: Aspidytidae). Zoological Journal of the Linnean Society 176: 537-546.