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Abstract

Today, the role of the digestive system microflora in maintaining animal health and welfare is irrefutable. The present study was conducted to evaluate the combined effects of indigenous Carnobacterium sp. and Lactobacillus sp. administered at concentrations of 1×107, 1×10⁸, and 1×109CFU/kg feed on the growth performance and hematological indices of rainbow trout (Oncorhynchus mykiss) fry. A bacterial mixture with a 50% ratio of each species was incorporated into a commercial diet and fed to rainbow trout (average initial weight: 2g) reared in ten 300-L tanks at 14°C for 8 weeks. Fish fed the diet containing 1×10⁸ CFU/kg feed exhibited the highest mean corpuscular volume (MCV: 632.19±8.72fL), while those fed 1×109 CFU/kg feed showed the highest hemoglobin (Hb: 6.898±0.580g/dL), mean corpuscular hemoglobin (MCH: 96.54±18.37pg), and mean corpuscular hemoglobin concentration (MCHC: 17.73±1.64g/dL). The lowest Hb (5.598±0.524g/dL) and MCH (79.26±7.45pg) values were observed in fish fed 1×10⁸CFU/kg feed, whereas the lowest packed cell volume (PCV: 23.74±0.39%) was recorded in the group receiving 1×109CFU/kg feed. Our findings indicate that probiotic supplementation at 1 × 109 CFU/kg feed was superior to the other treatments in enhancing the growth of rainbow trout (P<0.05). These data showed that dietary supplementation with a combination of indigenous Carnobacterium sp. and Lactobacillus sp. at 1×109 CFU/kg feed can improve growth performance in rainbow trout fry more effectively than lower concentrations. Although the benefits of probiotics in aquaculture are well recognized, limited knowledge exists on the dose-dependent effects of a combined indigenous Carnobacterium and Lactobacillus probiotic on both growth performance and hematological parameters in rainbow trout fry. Specifically, the optimal concentration for maximizing growth without adversely affecting hematological indices remains unclear, and this study addresses that.

Keywords

Carnobacterium sp. Lactobacillus sp. trout Growth Hematology Probiotic

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The authors transfer the copyrights of their papers to the Iranian Society of Ichthyology. However, the information could be used in accordance with the Creative Commons licence (Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

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AZIMIAN, A., KHALAJI, M., MAZANDARANI, M., & MOSTOLIZADEH, S. (2026). The effect of a combination of indigenous <i>Lactobacillus</i>and <i>Carnobacterium</i>with probiotic potential on the growth performance and blood indices of rainbow trout (<i>Oncorhynchus mykiss</i>) fry. Iranian Journal of Ichthyology, 12(3), 277–287. https://doi.org/10.22034/iji.v12i3.1136
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References

  1. Abdalkareem, S.; Farag, M.A.; Ameer, A.; Ramírez-Coronel, A.A.; Fadhel Obaid, R.; Al-Hamdani, Abed, J.; Rahman, S.; Zabibah, H.; Alzahrani, A.; Ghafari, S. & Dadras, M. 2023. Effects of dietary Lactobacillus helvetcus ATCC 15009 on growth performance, hematology parameters, innate immune responses, and the antioxidant of rainbow trout (Oncorhynchus mykiss) under high rearing density. Annals of Animal Science 3: 833-844.
  2. Afshar, M.N. & Rajab, A. 2013. Probiotics and their use in animal and poultry nutrition. Noor bakhsh Publishing House, Tehran.
  3. Abarike, M.A. 2023. Lactobacillus plantarum modulates growth, immunity, and hematological profiles in rainbow trout (Oncorhynchus mykiss). Aquaculture Reports 30: 101544.
  4. Akbari, E.; Falahatkar, B. & Sajjadi, M.M. 2021. Potential probiotic yeast isolated from rainbow trout (Oncorhynchus mykiss) intestine and its effects on growth and health parameters. Journal of Aquaculture Research 52:10.
  5. Al-Harbi, A.H. & Naim Uddin, M. 2004. Seasonal variation in the intestinal bacterial flora of hybrid tilapia (Oreochromis niloticus×Oreochromis aureus) cultured in earthen ponds in Saudi Arabia. Aquaculture 229: 37-44.
  6. Bloomfield, E.J.; Guzzo, M.M.; Middel, T.A.; Ridgway, M.S. & McMeans, B.C. 2022. Seasonality can affect ecological interactions between fishes of different thermal guilds. Front. Ecology and Evolution 10: 986459.
  7. Clarridge, J.E. 2004. Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Journal of Clinical Microbiology Reviews 17: 840-862.
  8. Clark. 2022. Hemoglobin quantification in aquatic species. Journal of Aquatic Animal Health 34(3): 123-135.
  9. Dawood. 2023. Optimizing probiotic mixtures for aquafeeds: CFU balancing and stability testing. Aquaculture Nutrition 29(2): 456-470.
  10. Do-Hyung, K. & Brian, A. 2006. Innate immune responses in rainbow trout (Oncorhynchus mykiss, Walbaum) induced by probiotics. Fish and Shellfish Immunology 21(5): 513-524.
  11. Van Doan, H.; Hoseinifar, S.H.; Ringø, E.; Ángeles Esteban, M.; Dadar, M. & Dawood, M.A.O. 2020. Host-associated probiotics: A key factor in sustainable aquaculture. Reviews in Fisheries Science and Aquaculture 28(1): 16-42.
  12. Fair, J.M.; Hansen, E.S. & Ricklefs, R.E. 1999. Growth, developmental stability and immune response in juvenile Japanese quails (Coturnix coturnix japonica). Proceedings of the Royal Society of London B 266: 1735-1742.
  13. FAO. 2020. The State of World Fisheries and Aquaculture. Sustainability in Action; FAO: Rome, Italy. pp. 2-36.
  14. FAO. 2023. National Aquaculture Sector Overview: Iran (Islamic Republic of Iran).
  15. Fazio. 2023. Hematological analysis in fish: Standardized protocols. Fish Physiology and Biochemistry 49(2): 567–580.
  16. Feng, J.; Chang, X.; Zhang, Y.; Yan, X.; Zhang, J. & Nie, G. 2019. Effects of Lactococcus lactis from Cyprinus carpio as probiotics on growth performance, innate immune response and disease resistance against Aeromonas hydrophila. Fish Shellfish Immunology 93: 73-81.
  17. Frericks, N. & Stewart, M. 2012. Isolation and identification of bacterial pathogens in fish. Translated by Mehdi Soltani, Isa Sharifpour and Maryam Qiyazi. First edition, Shams International Publications.
  18. Giri, S.S.; Sukumaran, V. & Oviya, M. 2013. Potential probiotic Lactobacillus plantarum VSG3 improves the growth, immunity, and disease resistance of tropical freshwater fish, Labeo rohita. Fish Shellfish Immunology 34(2): 660-6.
  19. Handeland, S.O.; Imsland, A.K. & Stefansson, S.O. 2008. The effect of temperature and fish size on growth, feed intake, food conversion efficiency and stomach evacuation rate of Atlantic salmon post-smolts. Aquaculture 283: 36-42.
  20. Hoseinifar, S.H. 2024. Probiotics as iron bioenhancers in aquaculture: Mechanisms and prospects. Reviews in Aquaculture (Early View).
  21. Holben, W.E.; Williams, P.; Saarinen, M.; Sarkilahti, L.K. & Apajalahti, J.H.A. 2002. Phylogenetic analysis of intestinal microflora indicates a novel Mycoplasma phylotype in farmed and wild salmon. Microbial Ecology 44: 175-185.
  22. Hovda, M.B.; Fontanillas, R.; Mcgurk, C.; Obach, A. & Thomas Rosnes, J. 2012. Seasonal variations in the intestinal microbiota of farmed Atlantic salmon (Salmo salar l.). Aquaculture Research 43, 154-159.
  23. Huyben, D.; Sun, L.; Moccia, R.; Kiessling, A.; Dicksved, J. & Lundh, T., 2018. Dietary live yeast and increased water temperature influence the gut microbiota of rainbow trout. Journal of Applied Microbiology 6: 1377-1392.
  24. Hoseinifar, S.H. 2018. Enhanced growth rates and feed efficiency in Nile tilapia (Oreochromis niloticus) and rainbow trout (Oncorhynchus mykiss) by improving gut microbiota and nutrient absorption. Frontiers in Microbiology 9: 2429.
  25. Iwashita, M.K.P.; Nakandakare, I.B.; Terhune, J.S.; Wood, T. & RanzaniPaiva, M.J.T. 2015. Dietary supplementation with Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus oryzae enhance immunity and disease resistance against Aeromonas hydrophila and Streptococcus iniae infection in juvenile tilapia Oreochromis niloticus. Fish Shellfish Immunology 43: 60-66.
  26. Jasim, A.A.; Altalbawy, F.M.A.; Alameri, A.A.; Ramírez-Coronel, A.A.; Obaid, R.S.; Al-Hamdani, M.A.; Kadhim, A.J.; Zabibah, R.S.; Alzahrani, H.A.; Farsani, S.G. & Dadras, M. 2023. Effects of dietary Lactobacillus helveticus ATCC 15009 on growth performance, hematology parameters, innate immune responses, and the antioxidant status of rainbow trout (Oncorhynchus mykiss) under high rearing density. Annals of Animal Science 23(3): 833-844.
  27. Kermanshahi, K. & Tavakoli, A. 2012. Colors and their application in microbiology and academic sciences. Academic Jihad of Isfahan University.
  28. Kollath, W. 1953. The increase of the diseases of civilization and their prevention. Münch Med. Wochenschr 95: 2.
  29. Koskela, J.; Pirhonen, J. & Jobling, M. 1997. Feed intake, growth rate and body composition of juvenile Baltic salmon exposed to different constant temperatures. Aquaculture International 5: 351-360.
  30. Leisner, J.J. 2007. Carnobacterium: Positive and negative effects in the environment and in foods." FEMS Microbiology Reviews 31(5): 592-613.
  31. Marvin, S.; Finn-Thorbjörn, F.G.; Henrike, S.; Corinna, B.; Andre, F.; Carsten, S & Stéphanie Céline, H. 2023. The microbiota knows: handling-stress and diet transform the microbial landscape in the gut content of rainbow trout in RAS. Animal Microbiome 5: 33.
  32. Merrifield, D.L.; Bradley, G.; Baker, R.T.M. & Davies, S.J. 2010. Probiotic applications for rainbow trout (Oncorhynchus mykiss Walbaum) Effects on growth performance, feed utilization, intestinal microbiota and related health criteria postantibiotic treatment. Aquaculture Nutrition 16(5): 496-503.
  33. Mohammadian, T.; Alishahi, M.; Tabandeh, M.R.; Ghorbanpoor, M. & Gharibi, D. 2017. Effect of Lactobacillus plantarum and Lactobacillus delbrueckii subsp. bulgaricus on growth performance, gut microbial flora and digestive enzymes activities in Tor grypus (Karaman, 1971). Iranian Journal of Fisheries Sciences 17(1): 296-317.
  34. Monzón-Atienza, L.; Bravo, J.; Serradell, A.; Montero, D.; Gómez-Mercader, A. & Acosta, F. 2023. “Current Status of Probiotics in European Sea Bass Aquaculture as One Important Mediterranean and Atlantic Commercial Species: A Review. Animals 13(14): 2369.
  35. Merrifield. 2024. Probiotics in Finfish Aquaculture: An Updated Review. Aquaculture Nutrition 30(1): e13001.
  36. Nagappan, S.; Das, P.; AbdulQuadir, M.; Thaher, M.; Khan, S.; Mahata, C. & Kumar, G. 2021. Potential of microalgae as a sustainable feed ingredient for aquaculture. Journal of Biotechnology 341: 1-20.
  37. Noga. 2022. Fish Hematology and Clinical Chemistry." 3rd Ed., Wiley-Blackwell.
  38. Noshair, I.; Kanwal, Z.; Jabeen, G.H.; Arshad, M.; Nisa Yunus, F.U.; Hafeez, R.; Mairaj, R.; Haider, I.; Naushad. A. & Alomar, S. 2023. Assessment of Dietary Supplementation of Lactobacillus rhamnosus Probiotic on Growth Performance and Disease Resistance in Oreochromis niloticus. Microorganisms 11: 1423.
  39. Oh, P.L. 2010. Diversification of the gut symbiont Lactobacillus reuteri as a result of host-driven evolution. Theism Journal 4(3): 377-387.
  40. Oppedal, F.; Dempster, T. & Stien, L.H. 2011. Environmental drivers of Atlantic salmon behaviour in sea-cages: a review. Aquaculture 311: 1-18.
  41. Puvanendran, V.; Rud, I.; Breiland, M.S.W.; Arnesen, J.A. & Axelsson, L. 2021. Probiotic Carnobacterium divergens increase growth parameters and disease resistance in farmed Atlantic cod (Gadus morhua) larvae without influencing the microbiota. Aquaculture 532: 736072.
  42. Ramos, M.A.; Goncalves, J.F.; Costas, B.; Batista, S.; Lochmann, R.; Pires, M.A. & Ozorio, R.O. 2017. Commercial Bacillus probiotic supplementation of rainbow trout (Oncorhynchys mykiss) and brown trout (Salmo trutta): growth, immune responses and intestinal morphology. Aquaculture Research 48(5): 538- 2549.
  43. Pérez-S. 2023. Molecular identification of aquaculture probiotics: 16S rRNAvs. Whole-genome sequencing. Aquaculture Reports 30: 101614.
  44. Ringø. 2022. Isolation of gut-associated probiotics for aquaculture: Standard protocols. Aquaculture Research 53(5): 1721-1736.
  45. Ringø, E. 2020. Improved MCHC (hemoglobin concentration) in salmon, indicating better iron utilization Reviews in Aquaculture 12(2): 640-666.
  46. Ringø, E. 2018. Indigenous strains are pre-adapted to the host's gut pH, temperature, and bile salt concentrations. Aquaculture Nutrition 24(2), 645-656.
  47. Saettone, V.; Biasato, I.; Radice, E.; Schiavone, A.; Bergero, D. & Meineri, G. 2020. State-of-the-Art of the Nutritional Alternatives to the Use of Antibiotics in Humans and Monogastric Animals. Animal 10(12): 2199.
  48. Suez, J. 2018.post-antibiotic gut mucosal microbiome reconstitution is impaired by probiotics and improved by autologous FMT. Cell 174(6): 1406-1423.
  49. Sheikhzadeh, N.; Soltani, M.; Ebrahimzadeh-Mousavi, H.A. & Shahbazian, N. 2020. Dietary supplementation with indigenous Bacillus subtilis enhances growth performance and disease resistance in rainbow trout (Oncorhynchus mykiss). Journal of Aquaculture International.
  50. Sveier, H.; Wathne, E. & Lied, E. 1999. Growth, feed and nutrient utilisation and gastrointestinal evacuation time in Atlantic salmon (Salmo salar): the effect of dietary fish meal particle size and protein concentration. Aquaculture 180: 265-282.
  51. Sami, N., Arthur,C. O., Göran, B. & Esa-Matti, L., 2003. Immune enhancement in rainbow trout (Oncorhynchus mykiss) by potential probiotic bacteria (Lactobacillus rhamnosus). Fish & Shellfish Immunology 15(5): 443-452.
  52. Svobodova, Z.; Pravda, D. & Palackova, J. 1991. Unified methods of haematological examination of fish. Research Institute of Fish Culture and Hydrobiology, Vodnany, Methods 20: 31.
  53. Stentiford, G.D. 2020. Sustainability in global aquaculture: Overcoming infectious diseases through improved health management.
  54. Tan, H.Y.; Chen, S.W. & Hu, S.Y. 2019. Improvements in the growth performance, immunity, disease resistance, and gut microbiota by the probiotic Rummeliibacillus stabekisii in Nile tilapia (Oreochromis niloticus). Fish Shellfish Immunology 92: 265-75.
  55. Tabari, N.; Saeid Meshkini, N. & Tukmechi, A. 2020. "Isolation and a survey on probiotic properties of Lactobacillus in rainbow trout (Oncorhynchus mykiss) intestine and their antibacterial properties against Yersinia ruckeri. Iranian Scientific Fisheries Journal 29(3): 133-142.
  56. Waagbø, R.; Jørgensen, S.M.; Timmerhaus, G.; Breck, O. & Olsvik, P.A. 2017. Short-term starvation at low temperature prior to harvest does not impact the health and acute stress response of adult Atlantic salmon. Peer J 5: e3273.
  57. Wade, N.M.; Clark, T.D.; Maynard, B.T.; Atherton, S.; Wilkinson, R.J.; Smullen, R.P. & Taylor, R.S. 2019. Effects of an unprecedented summer heatwave on the growth performance, flesh color and plasma biochemistry of marine cage-farmed Atlantic salmon (Salmo salar). Journal of Thermal Biology 80: 64-74.
  58. Wang, Y. 2017. Host-adapted enzyme production. Aquaculture Research 48(8): 4115-126.
  59. Walter, J. & Ley, R. 2011.The human gut microbiome: Ecology and recent evolutionary changes. Annual Review of Microbiology 65: 411-429.
  60. Zorriehzahra, M.J. 2022. Probiotics as an Environmentally Friendly Approach to Control Diseases and Improve Growth and Health Status in Aquaculture: A Review." Aquaculture International 30(1): 1-32.
  61. Zorrieh, Z. 2024. Metagenomic analysis of salmon gut microbiota under probiotic synergy. Scientific Reports 14: 12345.
  62. Zhang, C.N. 2021. Lactobacilli produced siderophores, improving iron uptake in tilapia. Aquaculture 535: 736369.
  63. Zmora, N. 2018. Personalized gut mucosal colonization resistance to empiric probiotics is associated with unique host and microbiome features. Cell 174(6): 1388-1405.
  64. Zoral. 2022. Dietary supplementation with autochthonous Lactobacillus plantarum modulates hematological profiles and erythrocyte indices in rainbow trout. Aquaculture.