TY - JOUR AU - AL-TEMEME, Tamadher M.K. AU - ABBAS, Basil A. PY - 2022/04/22 Y2 - 2024/03/29 TI - Antibiotic resistance and plasmid profiling of Pseudomonas aeruginosa isolated from some ruminants in Basrah, Iraq JF - Iranian Journal of Ichthyology JA - Iran. J. Ichthyol. VL - 9 IS - 0 SE - Articles DO - UR - http://www.ijichthyol.org/index.php/iji/article/view/819 SP - 334-339 AB - <p>This work aimed to determine the antibiotic-resistant patterns of<em> Pseudomonas&nbsp;aeruginosa</em> isolates obtained from clinical, healthy, and environmental samples from some ruminants (cows, and sheep). A total of 200 <em>P. aeruginosa </em>were obtained, and 52 isolates resisted all antibiotics used in the antibiotic sensitivity test. The antibiotic-resistant pattern showed that <em>P.</em> <em>aeruginosa</em> had high resistance (100%) to ampicillin, ceftazidime, gentamycin, ciprofloxacin, piperacillin, tobramycin, imipenem, amikacin, streptomycin, levofloxacin, rifampin, tetracycline, trimethoprim, ofloxacin, carbenicillin, penicillin, and nalidixic acid and had low resistant to colistin and fosfomycin. The plasmid profile was carried out on 12 selected multidrug-resistant (MDR) isolates that were resistant to more classes of antibiotics. All strains were found to possess plasmid bands. Five of the strains had 3 plasmid bands, 4 strains 2 plasmid bands and 3 strains possessed a single band. The sizes of the plasmids among <em>P. aeruginosa</em> isolates were 735, 1400 and 3000bp. All the strains that had plasmids were resistant to gentamycin, ciprofloxacin, piperacillin, tobramycin, imipenem, carbenicillin and tetracycline.</p> ER -