Morphological diversity of Garra rufa (Heckel, 1843) populations in Iran



In this study 485 specimen of Garra rufa (Heckel, 1843) from 29 populations from six river systems and basins including Tigris, Karkheh, Karun, Persis (Helleh and Mond), Hormuz and Maharlu were collected by a 5mm mesh sized seine net, anesthetized in 1% clove oil solution, fixed in 10% formalin and transferred to the laboratory for further investigations. Some 28 morphometric, 25 morphometric proportion and 10 meristic traits were examined. Different mean comparison methods, parametric and non-parametric analyses were used for statistical analyses. In comparison of the six aforementioned basins, for the proportions, Kruskal-Wallis non-parametric test and analysis of variance (ANOVA) for all the other traits, but the ratios of dorsal fin base/SL and pectoral fin/SL, showed significant differences (P<0.05). Amongst the meristic traits, except the number of vertebrae, caudal fin rays and pharyngeal teeth, all other traits were significantly different. Based on the compared data, none of the populations could be distinguished from each other.


Cyprinidae, Meristics, Morphometrics, Morphology, Doctor fish.

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Abdoli, A. 2000. The Inland Water Fishes of Iran. Iranian Museum of Nature & Wildlife, Tehran, Iran. (In Farsi)

Abedi, M.; Shiva, A.H. & Mohammadi, H. 2011. Reproductive biology and age determination of Garra rufa Heckel, 1843 (Actinopterygii: Cyprinidae) in central Iran. Turkish Journal of Zoology 35(3): 317-323.

Berg, L.S. 1949. Freshwater fishes of Iran and adjacent countries. Trudy Zoologicheskogo Instituta Akademii Nauk SSSR 8: 783-858.

Bianco, P.G. & Banarescu, P. 1982. A contribution to the knowledge of the Cyprinidae of Iran (Pisces, Cypriniformes). Cybium 6(2): 75-96.

Brinsmead, J. & Fox, M.G. 2002. Morphological variation between lake- and stream-dwelling rock bass and pumpkinseed populations. Journal of Fish Biology 61: 1619-1638.

Coad, B.W. 1998. Systematic biodiversity in the freshwater fishes of Iran. Italian Journal of Zoology 65: 101-108.

Coad, B.W. 2015. Freshwater fishes of Iran. (accessed 1 May 2015).

Daneshvar, E.; Keivany, Y. & Paknehad, E. 2013. Comparative biometry of the Iranian Cichlid, Iranocichla hormuzensis, in different seasons and sexes. Research in Zoology 3(2): 56-61.

Douglas, M.E. & Matthews, W.J. 1992. Does morphology predict ecology? hypothesis testing within a freshwater stream fish assemblage. Oikos 65: 213-224.

Durna, S.; Bardakci, F. & Degerli, N. 2009. Genetic diversity of Garra rufa Heckel, 1843 (Teleostei: Cyprinidae) in Anatolia. Biochemical Systematics and Ecology 20: 1-10.

Eagderi, S.; Esmaeilzadegan, E. & Pirbeigi, A. 2014. Morphological responses of Capoeta gracilis and Alburnoides eichwaldii populations (Cyprinidae) fragmented due to Tarik Dam (Sefidrud River, Caspian Sea basin, Iran). Iranian Journal of Ichthyology 1(2): 114-120.

Esmaeili, H.R. & Ebrahimi, M. 2006. Length-weight relationships of some freshwater fishes of Iran. Journal of Applied Ichthyology 22: 328-329.

Froese, R. & Pauly, D. 2015. FishBase, World Wide Web Electronic Publication,

Ghalenoei, M.; Pazooki, J.; Abdoli, A.; Hassanzadeh Kiabi, B. & Golzarian, K. 2010. Morphometric and meristic study of Garra rufa populations in Tigris and Persian Gulf basins. Iranian Scientific Fisheries Journal 19(3): 107-118. (In Farsi)

Gozukara, S.E. & Cavas, T. 2004. A karyological analysis of Garra rufa (Heckel, 1843)(Pisces, Cyprinidae) from the Eastern Mediterranean River basin in Turkey. Turkish Journal of Veterinary and Animal Sciences 28: 497-500.

Heckel, J.J. 1843. Ichthyologie. In: Russegger, J. (ed.), Reisen in Europa, Asien und Afrika, mit besonderer Rücksicht auf die naturwissenschaftlichen Verhältnisse der betreffenden Länder, unternommen in den Jahren 1835 bis 1841 von Joseph Russegger. Schweitzerbart'sche Verlagsbuchhandlung, Stuttgart 1(2): 991-1099.

Heidari, A.; Khoshkholgh, M. & Mousavi-Sabet, H. 2014. Tracing the effects of Sefidrud dams on Capoeta gracilis (Cyprinidae) populations using Truss distances in southern Caspian Sea basin. Iranian Journal of Ichthyology 1(2): 106-113.

Keivany, Y.; Nelson, J.S. & Economidis, P.S. 1997. Validity of Pungitius hellenicus, a stickleback fish from Greece. Copeia 1997(3): 558-564.

Keivany, Y.; Nasri, M.; Abbasi, K. & Abdoli, A. 2015. Atlas of Inland Water Fishes of Iran. Iran Department of Environment Press. (In press)

Khara, H.; Keyvan, A.; Vosoughi , G.; Pourkazemi, M.; Rezvani, S.; Nezami, S.A.; Ramin, M. & Sarpanah, A.N. 2006. Comparison of morphometric and meristic of bream (Abramis brama orientalis Berg 1905), in Caspian Sea and Anzali Wetland. Pajouhesh and Sazandegi (73): 177-187. (In Farsi)

Krupp, F. 1985. Systematik und Zoogeographie der Süßwasserfische des levantinischen Grabenbruchsystems und der Ostküste des Mittelmeeres. Dissertation zur Erlangung des Grades "Doktor der Naturwissenschaften" am Fachbereich Biologie der Johannes Gutenberg- Universität in Mainz. 215 pp., Anhang: Abbildungen, Karten, Tabellen, 169p.

Lavin, P.A. & McPhail, J.D. 1993. Parapatric lake and stream sticklebacks on northern Vancouver Island: disjunct distribution or parallel evolution. Canadian Journal of Zoology 71: 11-17.

Nezamoleslami, A.; Keivany, Y. & Dorafshan, S., 2015. Karyology of Garra rufa from Semirom River, Isfahan Province (Actinopterygii: Cyprinidae). Cell and Tissue: In press. (In Farsi)

Patimar, R. 2004. Inter- and intra-population diversity of Rutilus rutilus caspicus (Pravdin, 1928) in four wetlands in Golestan Province. PhD thesis, Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Iran. (In Farsi)

Patimar, R.; Chalanchi, M.G.; Chamanara, V. & Naderi, L. 2010. Some life history aspects of Garra rufa (Heckel, 1843) in the Kangir River, western Iran. Zoology in the Middle East 51: 57-66.

Rahemo, Z.I. 1995. Studies of the parasites of Garra tufa Heckel, 1843 (Pisces: Cyprinidae). Rivista Di Parassitologia 56: 273-278.

Rahmani, H. & Abdoli, A. 2008. Inter- population morphological diversity in Vimba vimba persa (Pallas, 1814) in Gorganrud River, Shirud River and Anzali lagoon. Journal of Agricultural Sciences and Natural Resources 15(1): 28-37. (In Farsi)

Rahmani, H. & Kiabi, B.H. 2006. Inter-population morphological diversity in Chalcalburnus chalcoides (Gueldenstaedt, 1772) in Haraz and Gazafrud rivers. Environmental Sciences 10: 21-34. (In Farsi)

Robinson, B.W. & Wilson, D.S. 1996: Genetic variation and phenotypic plasticity in a trophically polymorphic population of pumpkinseed sunfish (Lepomis gibbosus). Evolutionary Ecology 10: 631-652.

Samaee, S. M.; Patzner, R.A. & Mansour, N. 2009. Morphological differentiation within the population of Siah Mahi, Capoeta capoeta gracilis, (Cyprinidae, Teleostei) in a river of the south Caspian Sea basin: a pilot study. Journal of Applied Ichthyology 25: 583-590.

Thompson, C.E.; Taylor, E.B. & McPhail, J.D. 1997: Parallel evolution of lake-stream pairs of threespine sticklebacks (Gasterosteus) inferred from mitochondrial DNA variation. Evolution 51: 1955–1965.

Turan, C.; Yalcin, S.; Turan, F.; Okur, E. & Akyurt, I. 2005. Morphometric comparisons of African catfish, Clarias gariepinus, populations in Turkey. Folia Zoologica 54: 165-172.

Yazdanpanah, M. 2005. Reproductive biology of Garra rufa (Heckel, 1843) (Cypriniformes, Cyprinidae) in a spring-stream system, Zanjiran, Fars province. M.Sc. Thesis, Department of Biology, Shiraz University, Iran. (In Farsi)


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