Virulence gene expression of Pseudomonas aeruginosa isolated from children with nephrotic syndrome

Zainab Amer HATEM, Adnan Ali HAMMAD, Saade Abdalkareem JASIM


Pseudomonas aeruginosa is Gram-negative, considered as the most common opportunistic pathogens which lead to high rate of mortality and morbidity when subject suffered from compromised immune systems. This study was aimed to estimate the lasI and lasR genes expression of P. aeruginosa isolated from children with nephrotic syndrome. Nineteen isolates from urine samples of children with nephropathy syndrome are included in this study and confirmed by VITEK test as a P. aeruginosa strains. The result showed that the LasI and LasR genes showed a high level of gene expression in strains that grown with ceftriaxone than the strains that grown alone, but the gene LasI had a higher level of expression than the LasR gene, also a regression analysis were done to reveal the effect of LasI on the LasR gene which showed a positive effect which mean any increment in the gene expression level of LasI gene will combined with an increment in the LasR gene, (R= 0.899 and P= 0.001).


Nephrotic syndrome, LasI and LasR genes, Gene expression.

Full Text:



Asfahl, K.L. & Schuster, M. 2018. Additive effects of quorum sensing anti-activators on Pseudomonas aeruginosa virulence traits and transcriptome. Frontiers in Microbiology 8: 2654.

Cabrol, S.; Olliver, A.; Pier, G.B.; Andremont, A. & Ruimy, R. 2003. Transcription of Quorum-Sensing System Genes inClinical and Environmental Isolates of Pseudomonas aeruginosa. Journal of Bacteriology 185(24): 7222-7230.

Croda-García, G.; Grosso-Becerra, V.; Gonzalez-Valdez, A.; Servín-González, L. & Soberon-Chavez, G. 2011. Transcriptional regulation of Pseudomonas aeruginosa rhlR: role of the CRP orthologue Vfr (virulence factor regulator) and quorum-sensing regulators LasR and RhlR. Microbiology 157(9): 2545-2555.

Deschaght, P.; De Baets, F. & Vaneechoutte, M. 2011. PCR and the detection of Pseudomonas aeruginosa in respiratory samples of CF patients. A literature review. Journal of Cystic Fibrosis 10(5): 293-297.

Ding, F.; Oinuma, K.I.; Smalley, N.E.; Schaefer, A.L.; Hamwy, O.; Greenberg, E.P. & Dandekar, A.A. 2018. The Pseudomonas aeruginosa orphan quorum sensing signal receptor QscR regulates global quorum sensing gene expression by activating a single linked operon. MBio 9(4): e01274-18.

García-Contreras, R. 2016. Is quorum sensing interference a viable alternative to treat Pseudomonas aeruginosa infections? Frontiers in Microbiology 7: 1454.

Grosso-Becerra, M.V.; Santos-Medellín, C.; González-Valdez, A.; Méndez, J.L.; Delgado, G.; Morales-Espinosa, R. & Soberón-Chávez, G. 2014. Pseudomonas aeruginosa clinical and environmental isolates constitute a single population with high phenotypic diversity. BMC Genomics 15(1): 1-14.

Hemati, S.; Azizi-Jalilian, F.; Pakzad, I.; Taherikalani, M.; Maleki, A.; Karimi, S. & Sadeghifard, N. 2014. The correlation between the presence of quorum sensing, toxin-antitoxin system genes and MIC values with ability of biofilm formation in clinical isolates of Pseudomonas aeruginosa. Iranian Journal of Microbiology 6(3): 133.

Holmberg, C.; Antikainen, M.; Rönnholm, K.; Ala-Houhala, M. & Jalanko, H. 1995. Management of congenital nephrotic syndrome of the Finnish type. Pediatric Nephrology 9(1): 87-93.

Jamin, A.; Dehoux, L.; Dossier, C.; Fila, M.; Heming, N.; Monteiro, R.C. & Deschênes, G. 2015. Toll‐like receptor 3 expression and function in childhood idiopathic nephrotic syndrome. Clinical and Experimental Immunology 182(3): 332-345.

Jasim, S.A.; Feisal, H.T. & Abdulazeem L. 2020. A Review: Antimicrobial agent for pseudomonas aeruginosa isolated from Iraqi patients. Systematic Reviews in Pharmacy 11(12): 1117-1125.

Jasim, S.A. & Al-Abodi, H.R. 2021. Resistance rate and novel virulence factor determinants of Arcobacter spp., from cattle fresh meat products from Iraq. Microbial Pathogenesis 152: 104649.

Kidney International Supplements. 2011. Steroid-sensitive nephrotic syndrome in children. Kidney International Supplements 2(2): 163-171.

Köhler, T.; Perron, G.G.; Buckling, A. & Van Delden, C. 2010. Quorum sensing inhibition selects for virulence and cooperation in Pseudomonas aeruginosa. PLoS Pathogens 6(5): e1000883.

Kumar, L.; Brenner, N.; Brice, J.; Klein-Seetharaman, J. & Sarkar, S.K. 2020. Cephalosporins target quorum sensing and suppress virulence of Pseudomonas aeruginosa in Caenorhabditis elegans infection model. Biorxiv.

Lavenir, R.; Jocktane, D.; Laurent, F.; Nazaret, S. & Cournoyer, B. 2007. Improved reliability of Pseudomonas aeruginosa PCR detection by the use of the species-specific ecfX gene target. Journal of Microbiological Methods 70(1): 20-29.

Moghaddam, M.M.; Abolhassani, F.; Babavalian, H.; Mirnejad, R.; Barjini, K.A. & Amani, J. 2012. Comparison of in vitro antibacterial activities of two cationic peptides CM15 and CM11 against five pathogenic bacteria: Pseudomonas aeruginosa, Staphylococcus aureus, Vibrio cholerae, Acinetobacter baumannii, and Escherichia coli. Probiotics and Antimicrobial Proteins 4(2): 133-139.

Morales, E.; González-Valdez, A.; Servín-González, L. & Soberón-Chávez, G. 2017. Pseudomonas aeruginosa quorum-sensing response in the absence of functional LasR and LasI proteins: the case of strain 148, a virulent dolphin isolate. FEMS Microbiology Letters 364(12).

Naseri, M.; Naghibi, M.R. & Samezghandi, E. 2017. Pseudomonas Sepsis Associated with Skin and Cardiac Involvements in an Infant with Congenital Nephrotic Syndrome. Annals of Clinical Case Reports 2: 1286.

Rheault, M.N.; Zhang, L.; Selewski, D.T.; Kallash, M.; Tran, C.L. & Seamon, M. & Smoyer, W.E. 2015. AKI in children hospitalized with nephrotic syndrome. Clinical Journal of the American Society of Nephrology 10(12): 2110-2118.

Sandoz, K.M.; Mitzimberg, S.M. & Schuster, M. 2007. Social cheating in Pseudomonas aeruginosa quorum sensing. Proceedings of the National Academy of Sciences 104(40): 15876-15881.

Schuster, M.; Joseph Sexton, D.; Diggle, S.P. & Peter Greenberg, E. 2013. Acyl-homoserine lactone quorum sensing: from evolution to application. Annual Review of Microbiology 67: 43-63.

Senturk, S.; Ulusoy, S.; Bosgelmez-Tinaz, G. & Yagci, A. 2012. Quorum sensing and virulence of Pseudomonas aeruginosa during urinary tract infections. The Journal of Infection in Developing Countries 6(06): 501-507.

Siehnel, R.; Traxler, B.; An, D.D.; Parsek, M.R.; Schaefer, A.L. & Singh, P.K. 2010. A unique regulator controls the activation threshold of quorum-regulated genes in Pseudomonas aeruginosa. Proceedings of the National Academy of Sciences 107(17): 7916-7921.

Welsh, M.A.; Eibergen, N.R.; Moore, J.D. & Blackwell, H.E. 2015. Small molecule disruption of quorum sensing cross-regulation in Pseudomonas aeruginosa causes major and unexpected alterations to virulence phenotypes. Journal of the American Chemical Society 137(4): 1510-1519.

Youssef, D.M.; Abou Zeid, A.M.; Selim, M.S.A. & Sallam, M.M. 2019. Rate and type of infections in children with nephrotic syndrome. GEGET 14(1): 68-74.


  • There are currently no refbacks.